Qualitative and Quantitative Analysis of the Major Constituents in WLJ Herbal Tea Using Multiple Chromatographic Techniques.
Qualitative and Quantitative Analysis of the Major Constituents in WLJ Herbal Tea Using Multiple Chromatographic Techniques.
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采用多种色谱技术对王老吉凉茶中主要成分进行定性和定量分析
DOI：
10.3390/molecules23102623
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发表时间：
2018-10-12
期刊：
影响因子：
--
通讯作者：
Zhu CC
中科院分区：
文献类型：
--
作者：
Lin CZ;Zhang RJ;Yao YF;Huang XD;Zheng RB;Wu BJ;Zhu CC
中国凉茶的质量控制仍然是一个挑战，因为我们对其复杂的化学成分知之甚少。本研究采用全成分定量分析的方法，对中国畅销名牌饮料王老集凉茶的化学成分进行了研究。本文采用超高效液相色谱-四极杆串联飞行时间质谱仪(UPLC-Q-TOF-MS)对34种具有代表性的化学成分进行了鉴定和初步鉴定。此外，采用高效液相色谱-三重四级联用质谱仪(HPLC-MS/MS)对五味子中的14种成分进行了定量分析，并在Hilic色谱柱上采用高效液相色谱-蒸发光散射检测器(ELSD)分别对WLJHT的糖类组成进行了定量测定。多层析技术具有良好的精密度、灵敏度、重现性和稳定性，并成功地应用于16批WLJHT样品的分析。因此，该方法是一种可靠、实用的质量控制方法。
Quality control of Chinese herbal tea remains a challenge due to our poor knowledge of their complex chemical profile. This study aims to investigate the chemical composition of one of the best-selling and famous brand of beverage in China, Wanglaoji Herbal Tea (WLJHT), via a full component quantitative analysis. In this paper, a total of thirty-four representative constituents were identified or tentatively characterized using ultra-high performance liquid chromatography coupled with quadrupole tandem time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Moreover, the quantitative analyses of fourteen constituents were performed by high performance liquid chromatography with a triple quadruple tandem mass spectrometry (HPLC-MS/MS) method and saccharide compositions of WLJHT were also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on a Hilic column, separately. Using multiple chromatographic techniques presented a good precision, sensitivity, repeatability and stability, and was successfully applied to analyze 16 batches of WLJHT samples. Therefore, it would be a reliable and useful approach for the quality control of WLJHT.
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DOI：
10.1016/j.jpba.2009.02.023
发表时间：
2009-07-12
影响因子：
3.4
作者：
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作者：
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DOI：
10.3390/molecules21081092
发表时间：
2016-08-19
期刊：
Molecules (Basel, Switzerland)
影响因子：
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作者：
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通讯作者：
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