Silencing heat shock protein 27 decreases metastatic behavior of human head and neck squamous cell cancer cells in vitro.
Silencing heat shock protein 27 decreases metastatic behavior of human head and neck squamous cell cancer cells in vitro.
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DOI：
10.1021/mp100073s
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发表时间：
2010-08-02
影响因子：
4.9
通讯作者：
Sun D
中科院分区：
文献类型：
--
作者：
Zhu Z;Xu X;Yu Y;Graham M;Prince ME;Carey TE;Sun D
小热休克蛋白27 （Hsp27）是一种在癌细胞中参与多种细胞功能的分子伴侣。本研究的目的是研究Hsp27在头颈部鳞状细胞癌（HNSCC）细胞的体外转移行为。采用实时荧光定量PCR和western blotting检测Hsp27在原发性HNSCC和同一患者同步淋巴结转移的原代和转移细胞系中的表达。使用MTS增殖试验评估原发性和转移性HNSCC细胞系的增殖。通过迁移和侵袭试验评估转移行为。在高迁移性转移性HNSCC细胞系中进行了Hsp27的SiRNA敲低。MTS试验显示原发（UM-SCC-22A）和转移（UM-SCC-22B） HNSCC具有相似的增殖率。而转移源性UM-SCC-22B的迁移能力比UM-SCC-22A高2.3 ~ 3.6倍，侵袭能力比UM-SCC-22A高2倍。实时荧光定量PCR显示，转移性UM-SCC-22B中Hsp27 mRNA的表达是原发UM-SCC-22A的22.4倍。同样，Western blotting显示UM-SCC-22A中很少检测到Hsp27蛋白，而UM-SCC-22B表达的Hsp27蛋白水平高出25倍。sirna介导的UM-SCC-22B中Hsp27的敲低使Hsp27 mRNA表达减少近6倍，蛋白表达减少23倍。此外，Hsp27的siRNA敲低使UM-SCC-22B的转移行为在迁移方面降低了3 - 4倍，在细胞侵袭方面降低了2倍，使细胞侵袭和迁移降低到与原发HNSCC UM-SCC-22A相似的水平。这些数据表明，Hsp27可能调节HNSCC癌细胞的转移潜能。靶向Hsp27可减少头颈部鳞状细胞癌细胞的转移。
The small heat shock protein 27 (Hsp27) is a molecular chaperone that is involved in a variety of cellular functions in cancer cells. The purpose of this research is to study Hsp27 in vitro metastatic behaviors of head and neck squamous cell carcinoma cells (HNSCC). The expression of Hsp27 in primary and metastatic cell lines derived from the primary HNSCC and a synchronous lymph node metastasis in the same patient was determined using real-time PCR and western blotting. Proliferation of the primary and metastatic HNSCC cell lines was evaluated using the MTS proliferation assay. Metastatic behavior was assessed using migration and invasion assays. SiRNA knockdown of Hsp27 was performed in the highly migratory metastatic HNSCC cell line. MTS assays showed that the primary (UM-SCC-22A) and metastatic (UM-SCC-22B) HNSCC have similar proliferation rates. However, UM-SCC-22B derived from the metastasis showed 2.3 to 3.6-fold higher migration ability and 2-fold higher invasion ability than UM-SCC-22A. Real-time PCR demonstrated that Hsp27 mRNA is 22.4-fold higher in metastatic UM-SCC-22B than primary UM-SCC-22A. Similarly, Western blotting showed that Hsp27 is rarely detectable in UM-SCC-22A whereas UM-SCC-22B expresses a 25-fold higher level of Hsp27 protein. SiRNA-mediated knock down of Hsp27 in UM-SCC-22B reduced Hsp27 mRNA expression by nearly 6 - fold and protein expression by 23-fold. Furthermore, siRNA knockdown of Hsp27 decreased metastatic behaviors of UM-SCC-22B by 3 to 4-fold in migration and 2-fold in cell invasion reducing cell invasion and migration to levels similar to the primary HNSCC UM-SCC-22A. These data indicate that Hsp27 may regulate metastatic potential of HNSCC cancer cells. Targeting Hsp27 may decrease metastasis in head and neck squamous cell cancer cells.
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影响因子：
6
作者：
Hoang, AT;Huang, JP;Roy-Burman, P
通讯作者：
Roy-Burman, P
影响因子：
4.7
作者：
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通讯作者：
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影响因子：
11.5
作者：
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DOI：
10.1007/s00432-002-0357-y
发表时间：
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影响因子：
3.6
作者：
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通讯作者：
Papavassiliou, AG
影响因子：
2.1
作者：
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通讯作者：
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