Genomic Characteristics of Stx2e-Producing Escherichia coli Strains Derived from Humans, Animals, and Meats.
Genomic Characteristics of Stx2e-Producing Escherichia coli Strains Derived from Humans, Animals, and Meats.
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DOI：
10.3390/pathogens10121551
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发表时间：
2021-11-28
期刊：
影响因子：
--
通讯作者：
Bai X
中科院分区：
文献类型：
--
作者：
Yang X;Wu Y;Liu Q;Sun H;Luo M;Xiong Y;Matussek A;Hu B;Bai X
滋贺毒素（Stx）可分为Stx 1和Stx 2两种类型及不同的亚型。Stx 2 e是一种常见的引起猪水肿病的亚型，在人类中很少报道。本研究的目的是分析中国人源性产Stx 2 e大肠杆菌（Stx 2 e-STEC）菌株与动物源性和肉类来源菌株的流行情况和遗传特征。从我们的STEC收集中筛选Stx 2 e-STEC菌株，并进行全基因组测序以表征其遗传特征。我们的研究表明，Stx 2 e-STEC广泛分布在不同的宿主和较高的比例Stx 2 e-STEC在中国的人STEC菌株。3株人源Stx 2 e-STEC分离株分别属于O 100：H30、Onovel 26：H30和O 8：H9血清型，遗传特征各不相同。人Stx 2 e-STECs与动物和食物来源的菌株在遗传学上聚类。来自动物和肉类的Stx 2 e-STEC菌株表现出多重耐药，而人类菌株仅对阿奇霉素和四环素耐药。值得注意的是，高比例（55.9%）的Stx 2 e-STEC菌株，包括一种人类菌株，携带热稳定和热不稳定肠毒素编码基因st和lt，显示出STEC/肠毒素原性E.大肠杆菌（ETEC）杂交致病型。由于不同来源的Stx 2 e-STEC菌株没有明显的遗传特征，动物和食物来源的菌株可能会造成人类疾病的风险。
Shiga toxin (Stx) can be classified into two types, Stx1 and Stx2, and different subtypes. Stx2e is a subtype commonly causing porcine edema disease and rarely reported in humans. The purpose of this study was to analyze the prevalence and genetic characteristics of Stx2e-producing Escherichia coli (Stx2e-STEC) strains from humans compared to strains from animals and meats in China. Stx2e-STEC strains were screened from our STEC collection, and whole-genome sequencing was performed to characterize their genetic features. Our study showed a wide distribution of Stx2e-STEC among diverse hosts and a higher proportion of Stx2e-STEC among human STEC strains in China. Three human Stx2e-STEC isolates belonged to O100:H30, Onovel26:H30, and O8:H9 serotypes and varied in genetic features. Human Stx2e-STECs phylogenetically clustered with animal- and food-derived strains. Stx2e-STEC strains from animals and meat showed multidrug resistance, while human strains were only resistant to azithromycin and tetracycline. Of note, a high proportion (55.9%) of Stx2e-STEC strains, including one human strain, carried the heat-stable and heat-labile enterotoxin-encoding genes st and lt, exhibiting a STEC/enterotoxigenic E. coli (ETEC) hybrid pathotype. Given that no distinct genetic feature was found in Stx2e-STEC strains from different sources, animal- and food-derived strains may pose the risk of causing human disease.
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DOI：
10.1016/j.ijfoodmicro.2015.01.018
发表时间：
2015-05-04
影响因子：
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作者：
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影响因子：
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