Waste water effluent contributes to the dissemination of CTX-M-15 in the natural environment.
Waste water effluent contributes to the dissemination of CTX-M-15 in the natural environment.
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DOI：
10.1093/jac/dku079
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发表时间：
2014-07
期刊：
影响因子：
--
通讯作者：
Wellington EM
中科院分区：
文献类型：
--
作者：
Amos GC;Hawkey PM;Gaze WH;Wellington EM
耐多药肠杆菌科细菌对公众健康构成重大威胁。本研究旨在研究污水处理出水对河流中耐抗生素水库的影响。2009年和2011年，从污水处理厂(WWTP)的下游和上游采集了河流沉积物样本。对耐第三代头孢菌素(3GC)的肠杆菌科细菌进行了计数。用基于聚合酶链式反应的技术来阐明耐药机制，建立了一种新的基于两步聚合酶链式反应的方法来研究blaCTX-M-15动员。通过接合实验和不相容复制子分型来研究质粒生态学。我们报告了英国河流沉积物中blaCTX-M-15的第一个例子；blaCTX-M-15的流行率在污水处理厂下游显著增加。该基因的10种新的遗传背景被鉴定，携带在病原体如大肠杆菌ST131以及本土水生细菌如媒介气单胞菌中。BlaCTX-M-15基因可以很容易地转移到其他革兰氏阴性菌。我们还报告了在英国一条河流中首次发现耐亚胺培南的大肠杆菌。新遗传背景的高度多样性和寄主范围证明，新的抗性基因组合的进化发生的频率很高，到目前为止还被严重低估。我们已经在环境中发现了一个令人担忧的高度耐药的肠道细菌储存库，对人类和动物的健康构成了威胁。
Multidrug-resistant Enterobacteriaceae pose a significant threat to public health. We aimed to study the impact of sewage treatment effluent on antibiotic resistance reservoirs in a river. River sediment samples were taken from downstream and upstream of a waste water treatment plant (WWTP) in 2009 and 2011. Third-generation cephalosporin (3GC)-resistant Enterobacteriaceae were enumerated. PCR-based techniques were used to elucidate mechanisms of resistance, with a new two-step PCR-based assay developed to investigate blaCTX-M-15 mobilization. Conjugation experiments and incompatibility replicon typing were used to investigate plasmid ecology. We report the first examples of blaCTX-M-15 in UK river sediment; the prevalence of blaCTX-M-15 was dramatically increased downstream of the WWTP. Ten novel genetic contexts for this gene were identified, carried in pathogens such as Escherichia coli ST131 as well as indigenous aquatic bacteria such as Aeromonas media. The blaCTX-M-15 gene was readily transferable to other Gram-negative bacteria. We also report the first finding of an imipenem-resistant E. coli in a UK river. The high diversity and host range of novel genetic contexts proves that evolution of novel combinations of resistance genes is occurring at high frequency and has to date been significantly underestimated. We have identified a worrying reservoir of highly resistant enteric bacteria in the environment that poses a threat to human and animal health.
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DOI：
10.1016/j.diagmicrobio.2006.12.019
发表时间：
2007-06-01
影响因子：
2.9
作者：
Nhung, Pham Hong;Ohkusu, Kiyofumi;Ezaki, Takayuki
通讯作者：
Ezaki, Takayuki
影响因子：
4.9
作者：
Gaze, WH;Abdouslam, N;Wellington, EMH
通讯作者：
Wellington, EMH
影响因子：
4.4
作者：
Jechalke, Sven;Kopmann, Christoph;Smalla, Kornelia
通讯作者：
Smalla, Kornelia
影响因子：
14.9
作者：
Pilhofer M;Bauer AP;Schrallhammer M;Richter L;Ludwig W;Schleifer KH;Petroni G
通讯作者：
Petroni G
影响因子：
4.9
作者：
Humeniuk, C;Arlet, G;Philippon, A
通讯作者：
Philippon, A