Molecular analyses of the gill symbiosis of the bathymodiolin mussel Gigantidas platifrons.

Molecular analyses of the gill symbiosis of the bathymodiolin mussel Gigantidas platifrons.
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深莫迪林贻贝 Gigantidas platifrons 鳃共生的分子分析

DOI:
10.1016/j.isci.2020.101894
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发表时间:
2021-01-22
期刊:
影响因子:
5.8
通讯作者:
Li C
Li C
中科院分区:
综合性期刊2区
文献类型:
--
作者:
Wang H;Zhang H;Zhong Z;Sun Y;Wang M;Chen H;Zhou L;Cao L;Lian C;Li C

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尽管深海贻贝作为动物-细菌共生的模型已被广泛研究,但由于共生组织鳃的复杂性,评估宿主-共生体的相互作用仍然具有挑战性。以冷浸贻贝Gigantidas platifrons为模型,我们分离出共生体窝藏细菌细胞,并描绘了三个主要部分的共生-鳃,细菌细胞,和共生体的转录组。这种复杂的共生组织的分解使我们能够进一步表征宿主-共生体相互作用。我们的数据表明,鳃的非共生部分在维持和保护共生关系方面发挥着至关重要的作用;细菌细胞为共生体提供代谢产物,控制共生体种群,并保护共生体免受噬菌体感染;共生体致力于甲烷氧化和能源生产。这项研究表明,bathymodiolin共生在组织,细胞和分子水平上相互作用,保持高效率和和谐的化学合成微生态位。共生组织的分解表征深海贻贝共生细菌细胞为共生体提供代谢产物、庇护所和种群控制鳃的非共生部分在维持共生中也起着至关重要的作用贻贝和共生体共同努力维持高效的共生基因调控的分子机制;微生物基因组学;海水养殖
Although the deep-sea bathymodiolin mussels have been intensively studied as a model of animal-bacteria symbiosis, it remains challenging to assess the host-symbiont interactions due to the complexity of the symbiotic tissue—the gill. Using cold-seep mussel Gigantidas platifrons as a model, we isolated the symbiont harboring bacteriocytes and profiled the transcriptomes of the three major parts of the symbiosis—the gill, the bacteriocyte, and the symbiont. This breakdown of the complex symbiotic tissue allowed us to characterize the host-symbiont interactions further. Our data showed that the gill's non-symbiotic parts play crucial roles in maintaining and protecting the symbiosis; the bacteriocytes supply the symbiont with metabolites, control symbiont population, and shelter the symbiont from phage infection; the symbiont dedicates to the methane oxidation and energy production. This study demonstrates that the bathymodiolin symbiosis interacts at the tissue, cellular, and molecular level, maintaining high efficiency and harmonic chemosynthetic micro niche. Breakdown of the symbiotic tissue to characterize the deep-sea mussel symbiosis Bacteriocytes provide the symbiont with metabolites, shelter, and population control Non-symbiotic parts of the gill also play crucial roles in maintaining the symbiosis The mussel and the symbiont work together to maintain the high-efficiency symbiosis Molecular Mechanism of Gene Regulation; Microbial Genomics; Mariculture
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