Periostin as a novel biomarker for postoperative recurrence of chronic rhinosinitis with nasal polyps.
Periostin as a novel biomarker for postoperative recurrence of chronic rhinosinitis with nasal polyps.
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DOI：
10.1038/s41598-018-29612-2
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发表时间：
2018-07-30
影响因子：
4.6
通讯作者：
Fujieda S
中科院分区：
文献类型：
--
作者：
Ninomiya T;Noguchi E;Haruna T;Hasegawa M;Yoshida T;Yamashita Y;Okano M;Yoshida N;Haruna S;Sakuma Y;Ohta S;Ono J;Izuhara K;Okada M;Kidoguchi M;Tokunaga T;Okamoto M;Kanno M;Sakashita M;Takabayashi T;Narita N;Fujieda S
我们以前报道过慢性鼻-鼻窦炎鼻息肉(CRSwNP)根据JESREC评分系统被细分为四种慢性鼻-鼻窦炎(CRS)亚型。我们试图通过RNA序列鉴定与CRSwNP相关的基因表达谱和生物标志物。对6例CRSwNP患者的鼻息肉和下鼻甲黏膜的差异表达基因进行了RNA测序，并用实时定量聚合酶链式反应对结果进行了验证。用酶联免疫吸附试验确定可能的术后复发的生物标志物。在RNA测序结果中，Periostin(POSTN)在NP中的表达最高。我们以POSTN为研究对象，通过免疫组织化学和酶联免疫吸附试验检测POSTN的蛋白表达水平。免疫组织化学结果显示，POSTN在中、重度嗜酸性CRS中呈弥漫性表达，其染色模式与CRSwNP表型的严重程度相关(P <； 0.0 5)。当切断点为115.5 ng/ml时，POSTN高、低两组术后复发率差异有统计学意义(P<0.0072)。提示POSTN蛋白表达水平与CRSwNP的严重程度有关，血清POSTN可作为CRSwNP术后复发的一个新的生物标志物。
We previously reported that chronic rhinosinusitis with nasal polyps (CRSwNP) was subdivided into four chronic rhinosinusitis (CRS) subtypes using the JESREC scoring system. We sought to identify the gene expression profile and biomarkers related with CRSwNP by RNA-sequence. RNA-sequencing was performed to identify differentially expressed genes between nasal polyps (NPs) and inferior turbinate mucosa from 6 patients with CRSwNP, and subsequently, quantitative real-time PCR was performed to verify the results. ELISA was performed to identify possible biomarkers for postoperative recurrence. In the RNA-sequencing results, periostin (POSTN) expression was the highest in NP. We focused on POSTN and investigated the protein level of POSTN by immunohistochemistry and ELISA. POSTN was diffusely expressed in moderate and severe eosinophilic CRS using immunohistochemistry, and its staining pattern was associated with the severity of the phenotype of the CRSwNP (P < 0.05). There was a significant difference between the POSTN high/low groups for postoperative recurrence when the cutoff point was set at 115.5 ng/ml (P = 0.0072). Our data suggests that the protein expression level of POSTN was associated with the severity of CRSwNP, and serum POSTN can be a novel biomarker for postoperative recurrence of CRSwNP.
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DOI：
10.4168/aair.2011.3.3.186
发表时间：
2011-07
期刊：
Allergy, asthma & immunology research
影响因子：
--
作者：
Kimura S;Pawankar R;Mori S;Nonaka M;Masuno S;Yagi T;Okubo K
通讯作者：
Okubo K
影响因子：
14.2
作者：
Allakhverdi, Zoulfia;Comeau, Michael R.;Delespesse, Guy
通讯作者：
Delespesse, Guy
骨膜素：其在哮喘中的作用及其作为诊断或治疗靶点的潜力。
影响因子：
5.8
作者：
Li W;Gao P;Zhi Y;Xu W;Wu Y;Yin J;Zhang J
通讯作者：
Zhang J
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
14.2
作者：
Lee, Jae Yong;Lee, Sang Hag;Kang, Sun Mook
通讯作者：
Kang, Sun Mook