MOTHER-OF-FT-AND-TFL1 represses seed germination under far-red light by modulating phytohormone responses in Arabidopsis thaliana.
MOTHER-OF-FT-AND-TFL1 represses seed germination under far-red light by modulating phytohormone responses in Arabidopsis thaliana.
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DOI：
10.1073/pnas.1806460115
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发表时间：
2018-08-14
影响因子：
11.1
通讯作者：
Graham IA
中科院分区：
文献类型：
--
作者：
Vaistij FE;Barros-Galvão T;Cole AF;Gilday AD;He Z;Li Y;Harvey D;Larson TR;Graham IA
种子感知环境条件，如光质，以调节其发芽。光丰富的远红波长，如树冠下的光（阴影），增加脱落酸（ABA）和降低赤霉素（GA）的水平，植物激素抑制和促进发芽，分别。我们已经发现，MFT功能作为一个关键的抑制剂发芽远红光条件下，通过调节ABA和GA的反应。许多植物物种的种子萌发是由阳光引发的，阳光富含红色（R）波长，并被富含远红色（FR）的树冠下光抑制。R：FR比率通过光敏色素来调节赤霉素（GAs）和脱落酸（ABA）的水平，赤霉素和脱落酸分别诱导和抑制萌发。在这项研究中，我们发现，在FR光条件下，发芽被抑制的母亲-FT-和-TFL1（MFT）通过调节ABA和GA信号通路。我们还表明，MFT基因的表达是严格调控的光质量。先前的工作已经表明，在FR光条件下，转录因子Phyochrome-Interacting-Factor 1（PIF 1）积累并促进SOMNUS（SOM）的表达，这反过来导致ABA增加和GA水平降低。PIF1还促进编码ABA-不敏感5（ABI5）和DELLA生长抑制蛋白的基因的表达，这两种蛋白分别在ABA和GA信号通路中起作用。在这里，我们表明，MFT基因的表达是由FR光通过PIF1/SOM/ABI 5/DELLA途径促进，并通过转录因子SPATlets（SPT）由R光抑制。与此相一致，我们还表明，SPT基因的表达被抑制在FR光PIF1依赖的方式。此外，本研究中的转录组学分析表明，MFT通过促进已知ABA诱导基因的表达和抑制细胞壁扩张相关基因来发挥其功能。
Seeds sense environmental conditions, such as light quality, to regulate their germination. Light enriched in the far-red wavelength, such as under-the-canopy light (shade), increases abscisic acid (ABA) and decreases gibberellin (GA) levels, the phytohormones repressing and promoting germination, respectively. We have discovered that MFT functions as a key repressor of germination under far-red light conditions by modulating ABA and GA responses. Seed germination in many plant species is triggered by sunlight, which is rich in the red (R) wavelength and repressed by under-the-canopy light rich in far red (FR). R:FR ratios are sensed by phytochromes to regulate levels of gibberellins (GAs) and abscisic acid (ABA), which induce and inhibit germination respectively. In this study we have discovered that, under FR light conditions, germination is repressed by MOTHER-OF-FT-AND-TFL1 (MFT) through the regulation of the ABA and GA signaling pathways. We also show that MFT gene expression is tightly regulated by light quality. Previous work has shown that under FR light conditions the transcription factor PHYOCHROME-INTERACTING-FACTOR1 (PIF1) accumulates and promotes expression of SOMNUS (SOM) that, in turn, leads to increased ABA and decreased GA levels. PIF1 also promotes expression of genes encoding ABA-INSENSITIVE5 (ABI5) and DELLA growth-repressor proteins, which act in the ABA and GA signaling pathways, respectively. Here we show that MFT gene expression is promoted by FR light through the PIF1/SOM/ABI5/DELLA pathway and is repressed by R light via the transcription factor SPATULA (SPT). Consistent with this, we also show that SPT gene expression is repressed under FR light in a PIF1-dependent manner. Furthermore, transcriptomic analyses presented in this study indicate that MFT exerts its function by promoting expression of known ABA-induced genes and repressing cell wall expansion-related genes.
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