The Diversity, Resistance Profiles and Plasmid Content of Klebsiella spp. Recovered from Dairy Farms Located around Three Cities in Pakistan.
The Diversity, Resistance Profiles and Plasmid Content of Klebsiella spp. Recovered from Dairy Farms Located around Three Cities in Pakistan.
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DOI：
10.3390/antibiotics12030539
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发表时间：
2023-03-08
影响因子：
4.8
通讯作者：
Feil, Edward J. J.
中科院分区：
文献类型：
--
作者：
Habib, Samia;Gibbon, Marjorie J. J.;Couto, Natacha;Kakar, Khadija;Habib, Safia;Samad, Abdul;Munir, Asim;Fatima, Fariha;Mohsin, Mashkoor;Feil, Edward J. J.
肺炎克雷伯氏菌（Kp）等细菌病原体中抗菌素耐药性（AMR）的上升是一个紧迫的公共卫生和经济问题。“一个健康”框架认识到，有效管理抗生素耐药性需要在农业和临床环境中进行监测，特别是在巴基斯坦等低资源地区。在这里，我们使用全基因组测序的克雷伯氏菌属49株。（包括43 Kp）和2个假定的Providencia rettgeri分离株，它们来自巴基斯坦3个城市附近的奶牛场-奎达（n = 29）、费萨拉巴德（n = 19）和萨戈达（n = 3）。43株Kp分离株对应38种序列类型（ST），其中35种ST仅观察到一次。这种高多样性表明频繁的混合物和有限的克隆传播在当地规模。克雷伯氏菌属49种。分离株中，41株（84%）不含任何临床相关的抗菌素耐药基因（ARG），我们没有检测到任何预测编码碳青霉烯类或粘菌素耐药的ARG。然而，4个Kp谱系包含多个ARG：ST 11（n = 2）、ST 1391 - 1 LV（n = 1）、ST 995（n = 1）和ST 985（n = 1）。STs 11、1391- 1 LV和995共有一组核心的5个ARG，包括blaCTX-M-15，位于不同的AMR质粒上。ST 985携带不同的16个抗性基因，包括blaCTX-M-55。两种推定的雷氏毕赤酵母分离株也含有多种ARG。最后，在我们的数据集中没有携带ARG的四种最常见的质粒在区域之间非随机分布，这表明质粒的局部扩增独立于宿主细菌谱系发生。关于奶牛场如何促进AMR在巴基斯坦的出现和传播的证据对于负责卫生、农业和环境以及工业发展的公共当局和组织非常有价值。
The rise of antimicrobial resistance (AMR) in bacterial pathogens such as Klebsiella pneumoniae (Kp) is a pressing public health and economic concern. The ‘One-Health’ framework recognizes that effective management of AMR requires surveillance in agricultural as well as clinical settings, particularly in low-resource regions such as Pakistan. Here, we use whole-genome sequencing to characterise 49 isolates of Klebisella spp. (including 43 Kp) and 2 presumptive Providencia rettgeri isolates recovered from dairy farms located near 3 cities in Pakistan—Quetta (n = 29), Faisalabad (n = 19), and Sargodha (n = 3). The 43 Kp isolates corresponded to 38 sequence types (STs), and 35 of these STs were only observed once. This high diversity indicates frequent admixture and limited clonal spread on local scales. Of the 49 Klebsiella spp. isolates, 41 (84%) did not contain any clinically relevant antimicrobial resistance genes (ARGs), and we did not detect any ARGs predicted to encode resistance to carbapenems or colistin. However, four Kp lineages contained multiple ARGs: ST11 (n = 2), ST1391-1LV (n = 1), ST995 (n = 1) and ST985 (n = 1). STs 11, 1391-1LV and 995 shared a core set of five ARGs, including blaCTX-M-15, harboured on different AMR plasmids. ST985 carried a different set of 16 resistance genes, including blaCTX-M-55. The two presumptive P. rettgeri isolates also contained multiple ARGs. Finally, the four most common plasmids which did not harbour ARGs in our dataset were non-randomly distributed between regions, suggesting that local expansion of the plasmids occurs independently of the host bacterial lineage. Evidence regarding how dairy farms contribute to the emergence and spread of AMR in Pakistan is valuable for public authorities and organizations responsible for health, agriculture and the environment, as well as for industrial development.
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影响因子：
10.7
作者：
Price MN;Dehal PS;Arkin AP
通讯作者：
Arkin AP
在自然环境或临床环境中繁盛的肺炎克雷伯菌的共同和独特的基因组特征。
影响因子：
4.6
作者：
Rocha, Jaqueline;Henriques, Isabel;Gomila, Margarita;Manaia, Celia M.
通讯作者：
Manaia, Celia M.
影响因子：
3.7
作者：
Qamar A;Ismail T;Akhtar S
通讯作者：
Akhtar S
影响因子：
4.4
作者：
Schubert, Hannah;Morley, Katy;Reyher, Kristen K.
通讯作者：
Reyher, Kristen K.
影响因子：
3.9
作者：
Argimón S;Abudahab K;Goater RJE;Fedosejev A;Bhai J;Glasner C;Feil EJ;Holden MTG;Yeats CA;Grundmann H;Spratt BG;Aanensen DM
通讯作者：
Aanensen DM