Subchondral bone derived mesenchymal stem cells display enhanced osteo-chondrogenic differentiation, self-renewal and proliferation potentials.
Subchondral bone derived mesenchymal stem cells display enhanced osteo-chondrogenic differentiation, self-renewal and proliferation potentials.
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软骨下骨来源的间充质干细胞表现出增强的骨软骨分化、自我更新和增殖潜力。
DOI：
10.1538/expanim.17-0137
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发表时间：
2018-07-30
影响因子：
2.4
通讯作者：
Zhu H
中科院分区：
文献类型：
--
作者：
Zhang H;Li ZL;Su XZ;Ding L;Li J;Zhu H
兔骨髓间充质干细胞（MSCs）是再生医学研究，特别是转化研究的重要种子细胞。在目前的研究中，我们发现兔软骨下骨是一种可靠的MSCs来源。首先，我们从兔膝关节获取软骨下骨（SCB），通过培养酶处理的SCB来启动MSC培养。从SCB生长出来的贴壁成纤维细胞样细胞满足用于定义MSC的常见免疫表型标准，但具有低污染的CD 45+造血细胞。有趣的是，分化的SCB-MSC表达的成骨和成软骨标志物水平显着高于骨髓细胞悬液来源的MSC（BMS-MSC）（P<0.05）。SCB-MSC和BMS-MSC成脂标志物的表达差异无统计学意义（P>0.05）。殖民地集落形成单位-成纤维细胞实验和成球实验结果表明，SCB-MSCs具有较强的自我更新能力。SCB-MSCs的干性标志物Nanog、OCT 4和Sox-2的表达水平高于BMS-MSCs（P<0.05）。CCK-8法和CFSE法结果均显示SCB-MSCs具有较强的增殖能力。此外，SCB-MSCs表现出更高的细胞外信号相关激酶/丝裂原活化蛋白激酶信号的磷酸化，这与MSC增殖密切相关。总之，我们确定SCB-MSC作为一种新的干细胞群体，满足MSC的要求; SCB-MSC的独特性质是重要的潜在治疗疾病和创伤引起的组织损伤。
Rabbit mesenchymal stem cells (MSCs) are important seed cells in regenerative medicine research, particularly in translational research. In the current study, we showed that rabbit subchondral bone is a reliable source of MSCs. First, we harvested subchondral bone (SCB) from the rabbit knee-joint and initiated the MSC culture by cultivating enzyme-treated SCB. Adherent fibroblast-like cells that outgrew from SCB fulfill the common immuno-phenotypic criteria for defining MSCs, but with low contamination of CD45+ hematopoietic cells. Interestingly, differentiated SCB-MSCs expressed osteogenic and chondrogenic markers at significantly higher levels than those in bone marrow cell suspension-derived MSCs (BMS-MSCs) (P<0.05). No differences in the expression of adipogenic markers between SCB-MSC and BMS-MSC (P>0.05) were observed. Moreover, the results of the colony forming unit-fibroblast assay and sphere formation assay demonstrated that the SCB-MSCs had increased self-renewal potential. SCB-MSCs expressed higher levels of the stemness markers Nanog, OCT4, and Sox-2 compared to in BMS-MSCs (P<0.05). Furthermore, the results of both the CCK-8-based assay and CFSE dilution assay showed that SCB-MSCs exhibited enhanced proliferative capacity. In addition, SCB-MSCs exhibited higher phosphorylation of extracellular signal-related kinase/mitogen-activated protein kinase signaling, which is closely related to MSC proliferation. In conclusion, we identified SCB-MSCs as a novel stem cell population that met the requirements of MSCs; the unique properties of SCB-MSC are important for the potential treatment of tissue damage resulting from disease and trauma.
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影响因子：
2.5
作者：
Ilas DC;Churchman SM;McGonagle D;Jones E
通讯作者：
Jones E
DOI：
10.1016/j.bbrc.2014.01.084
发表时间：
2014-02-28
影响因子：
3.1
作者：
Eom, Young Woo;Oh, Ji-Eun;Shim, Kwang Yong
通讯作者：
Shim, Kwang Yong
影响因子：
5.2
作者：
Griffin, Matthew D.;Elliman, Stephen J.;Ritter, Thomas
通讯作者：
Ritter, Thomas
DOI：
10.1016/j.injury.2010.09.027
发表时间：
2010-11-01
影响因子：
2.5
作者：
de Girolamo, Laura;Bertolini, Giulia;Volpi, Piero
通讯作者：
Volpi, Piero
影响因子：
6.2
作者：
FRIEDENSTEIN, AJ;CHAILAKHYAN, RK;KEILISSB.IV
通讯作者：
KEILISSB.IV