Adenoviral vectored vaccination protects against Crimean-Congo Haemorrhagic Fever disease in a lethal challenge model.
Adenoviral vectored vaccination protects against Crimean-Congo Haemorrhagic Fever disease in a lethal challenge model.
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DOI：
10.1016/j.ebiom.2023.104523
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发表时间：
2023-04
期刊：
影响因子：
11.1
通讯作者：
Lambe, Teresa
中科院分区：
文献类型：
--
作者：
Saunders, Jack E.;Gilbride, Ciaran;Dowall, Stuart;Morris, Susan;Ulaszewska, Marta;Spencer, Alexandra J.;Rayner, Emma;Graham, Victoria A.;Kennedy, Emma;Thomas, Kelly;Hewson, Roger;Gilbert, Sarah C.;Belij-Rammerstorfer, Sandra;Lambe, Teresa
蜱传布尼亚病毒，即克里米亚-刚果出血热病毒（CCHFV），可在人类中引起严重的发热性疾病，具有广泛的地理范围，并且由于蜱的迁移而继续扩大。目前，针对CCHFV还没有获得许可的疫苗可以广泛使用。在这项研究中，我们描述了一种黑猩猩腺病毒载体疫苗（ChAdOx2 CCHF）的临床前评估，该疫苗编码CCHFV的糖蛋白前体（GPC）。我们在这里证明，接种ChAdOx2 CCHF可在小鼠中诱导体液和细胞免疫应答，并在致死性CCHF攻击模型中产生100%的保护。腺病毒疫苗在异源疫苗方案中与改良安卡拉牛苗（MVA CCHF）一起递送，在小鼠中诱导最高水平的cchfv特异性细胞介导和抗体反应。ChAdOx2 CCHF免疫小鼠的组织病理学检查和病毒载量分析显示，没有与CCHF感染相关的显微变化和病毒抗原，进一步证明了对疾病的保护。仍然需要一种有效的CCHFV疫苗，以保护人类免受致命的出血性疾病的侵害。我们的研究结果支持进一步开发表达CCHFV GPC的ChAd平台，以寻求针对CCHFV的有效疫苗。本研究由（UKRI-BBSRC） [BB/R019991/1和BB/T008784/1]资助。
The tick-borne bunyavirus, Crimean-Congo Haemorrhagic Fever virus (CCHFV), can cause severe febrile illness in humans and has a wide geographic range that continues to expand due to tick migration. Currently, there are no licensed vaccines against CCHFV for widespread usage. In this study, we describe the preclinical assessment of a chimpanzee adenoviral vectored vaccine (ChAdOx2 CCHF) which encodes the glycoprotein precursor (GPC) from CCHFV. We demonstrate here that vaccination with ChAdOx2 CCHF induces both a humoral and cellular immune response in mice and 100% protection in a lethal CCHF challenge model. Delivery of the adenoviral vaccine in a heterologous vaccine regimen with a Modified Vaccinia Ankara vaccine (MVA CCHF) induces the highest levels of CCHFV-specific cell-mediated and antibody responses in mice. Histopathological examination and viral load analysis of the tissues of ChAdOx2 CCHF immunised mice reveals an absence of both microscopic changes and viral antigen associated with CCHF infection, further demonstrating protection against disease. There is the continued need for an effective vaccine against CCHFV to protect humans from lethal haemorrhagic disease. Our findings support further development of the ChAd platform expressing the CCHFV GPC to seek an effective vaccine against CCHFV. This research was supported by funding from the (UKRI-BBSRC) [BB/R019991/1 and BB/T008784/1].
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影响因子：
5.5
作者：
Draper SJ;Cottingham MG;Gilbert SC
通讯作者：
Gilbert SC
DOI：
10.1056/nejmoa2105290
发表时间：
2021-12-16
期刊：
The New England journal of medicine
影响因子：
--
作者：
Falsey AR;Sobieszczyk ME;Hirsch I;Sproule S;Robb ML;Corey L;Neuzil KM;Hahn W;Hunt J;Mulligan MJ;McEvoy C;DeJesus E;Hassman M;Little SJ;Pahud BA;Durbin A;Pickrell P;Daar ES;Bush L;Solis J;Carr QO;Oyedele T;Buchbinder S;Cowden J;Vargas SL;Guerreros Benavides A;Call R;Keefer MC;Kirkpatrick BD;Pullman J;Tong T;Brewinski Isaacs M;Benkeser D;Janes HE;Nason MC;Green JA;Kelly EJ;Maaske J;Mueller N;Shoemaker K;Takas T;Marshall RP;Pangalos MN;Villafana T;Gonzalez-Lopez A;AstraZeneca AZD1222 Clinical Study Group
通讯作者：
AstraZeneca AZD1222 Clinical Study Group
影响因子：
5.5
作者：
Cottingham, Matthew G.;Carroll, Miles W.
通讯作者：
Carroll, Miles W.
DOI：
10.3390/v6072735
发表时间：
2014-07-17
期刊：
Viruses
影响因子：
--
作者：
Altenburg AF;Kreijtz JH;de Vries RD;Song F;Fux R;Rimmelzwaan GF;Sutter G;Volz A
通讯作者：
Volz A
影响因子：
2.1
作者：
Atkinson, Barry;Chamberlain, John;Hewson, Roger
通讯作者：
Hewson, Roger