Functional and morphologic dysfunctions in the airways of rats submitted to an experimental model of obesity-exacerbated asthma.
Functional and morphologic dysfunctions in the airways of rats submitted to an experimental model of obesity-exacerbated asthma.
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DOI：
10.1038/s41598-022-13551-0
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发表时间：
2022-06-09
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
肥胖加重哮喘表型的特征是更严重的哮喘症状和糖皮质激素抵抗。本研究的目的是通过高血糖水平指数（HGLI）饮食和卵清蛋白（OVA）致敏和刺激来标准化Wistar大鼠肥胖加重哮喘模型。将大鼠分为对照组（Ctrl）、肥胖组（Ob）、哮喘组（ash）、肥胖哮喘组（Ob + ash）和地塞米松治疗的肥胖哮喘组（Ob + ash + Dexa），测定其体内和体外功能和形态学参数。食用HGLI后，体重、空腹血糖、腹围、体重指数和肥胖指数均有所增加。呼吸功能表现为肺潮气量和通气量减少。在离体气管中，carbachol对Ob、Ob + ash和Ob + ash + Dexa的收缩效能均有增加，但主要对Ob + ash有增加作用。肺组织学分析显示Ob + Asth和Ob + Asth + Dexa的支气管血管周围炎症、平滑肌肥大和细胞外重塑。成功建立肥胖加重哮喘模型。因此，该模型允许进一步的分子研究和寻找治疗和缓解肥胖诱导的顽固性哮喘患者症状的新疗法。
The obesity-exacerbated asthma phenotype is characterized by more severe asthma symptoms and glucocorticoid resistance. The aim of this study was to standardize an obesity-exacerbated asthma model by a high glycemic level index (HGLI) diet and ovalbumin (OVA) sensitization and challenges in Wistar rats. Animals were divided into groups: control (Ctrl), obese (Ob), asthmatic (Asth), obese asthmatic (Ob + Asth) and obese asthmatic treated with dexamethasone (Ob + Asth + Dexa), and in vivo and in vitro functional and morphological parameters were measured. After HGLI consumption, there was an increase in body weight, fasting blood glucose, abdominal circumferences, body mass index and adiposity index. Respiratory function showed a reduction in pulmonary tidal volume and ventilation. In isolated tracheas, carbachol showed an increase in contractile efficacy in the Ob, Ob + Asth and Ob + Asth + Dexa, but mostly on Ob + Asth. Histological analysis of lungs showed peribronchovascular inflammation and smooth muscle hypertrophy and extracellular remodeling on Ob + Asth and Ob + Asth + Dexa. An obesity-exacerbated asthma model was successfully established. Therefore, this model allows further molecular investigations and the search for new therapies for the treatment and relief of symptoms of patients with obesity-induced resistant asthma.
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影响因子：
3
作者：
Alves, Adriano F.;Pereira, Ramon A.;Tafuri, Wagner L.
通讯作者：
Tafuri, Wagner L.
影响因子：
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作者：
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影响因子：
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影响因子：
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通讯作者：
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影响因子：
9.6
作者：
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