SLM produced porous titanium implant improvements for enhanced vascularization and osteoblast seeding.
SLM produced porous titanium implant improvements for enhanced vascularization and osteoblast seeding.
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DOI：
10.3390/ijms16047478
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发表时间：
2015-04-02
影响因子：
5.6
通讯作者：
Nolte I
中科院分区：
文献类型：
--
作者：
Matena J;Petersen S;Gieseke M;Kampmann A;Teske M;Beyerbach M;Murua Escobar H;Haferkamp H;Gellrich NC;Nolte I
为了改进众所周知的钛植入物，孔可用于增加骨形成并闭合骨-植入物界面。选择性激光熔化 (SLM) 可以生产任何几何形状，并用于生产孔径为 250 µm 的植入物。使用的孔径支持血管向内生长，因为骨形成强烈依赖于快速血管化。此外，促血管生成因子促进植入物血管化。为了使钛具有促血管生成因子功能化，可以使用聚己内酯（PCL）涂层。检查了以下促血管生成因子：血管内皮生长因子 (VEGF)、高迁移率族蛋白 1 (HMGB1) 和趋化因子（C-X-C 基序）配体 12 (CXCL12)。由于不同的表面导致不同的电池反应，因此对钛和PCL涂层进行了比较。通过横截面检查原代成骨细胞向多孔钛结构的生长。使用活细胞成像（LCI）对接种在不同表面上的原代成骨细胞进行比较。横截面显示细胞已增殖，但 7 天后未迁移。尽管 LCI 中钛 PCL 植入物的细胞计数较低，但细胞计数和细胞扩散区域的发展显示钛 PCL 植入物有希望的结果。 HMGB1 显示出刺激内皮细胞系的最高迁移能力。未来的前景是将HMGB1掺入PCL聚合物中以实现缓慢因子释放。
To improve well-known titanium implants, pores can be used for increasing bone formation and close bone-implant interface. Selective Laser Melting (SLM) enables the production of any geometry and was used for implant production with 250-µm pore size. The used pore size supports vessel ingrowth, as bone formation is strongly dependent on fast vascularization. Additionally, proangiogenic factors promote implant vascularization. To functionalize the titanium with proangiogenic factors, polycaprolactone (PCL) coating can be used. The following proangiogenic factors were examined: vascular endothelial growth factor (VEGF), high mobility group box 1 (HMGB1) and chemokine (C-X-C motif) ligand 12 (CXCL12). As different surfaces lead to different cell reactions, titanium and PCL coating were compared. The growing into the porous titanium structure of primary osteoblasts was examined by cross sections. Primary osteoblasts seeded on the different surfaces were compared using Live Cell Imaging (LCI). Cross sections showed cells had proliferated, but not migrated after seven days. Although the cell count was lower on titanium PCL implants in LCI, the cell count and cell spreading area development showed promising results for titanium PCL implants. HMGB1 showed the highest migration capacity for stimulating the endothelial cell line. Future perspective would be the incorporation of HMGB1 into PCL polymer for the realization of a slow factor release.
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影响因子：
9
作者：
Laschke, Matthias W.;Ruecker, Martin;Menger, Michael D.
通讯作者：
Menger, Michael D.
DOI：
10.1016/j.jmatprotec.2003.11.051
发表时间：
2004-06-10
影响因子：
6.3
作者：
Kruth, JP;Froyen, L;Lauwers, B
通讯作者：
Lauwers, B
影响因子：
4.9
作者：
Lindhorst, Daniel;Tavassol, Frank;Ruecker, Martin
通讯作者：
Ruecker, Martin
影响因子：
9.7
作者：
De Giglio, Elvira;Cometa, Stefania;Mattioli-Belmonte, Monica
通讯作者：
Mattioli-Belmonte, Monica
影响因子：
2.9
作者：
Niu, Xiaowei;Yang, Cuiling;Yao, Yali
通讯作者：
Yao, Yali