Morphometric and immunohistochemical study of cholangiolocellular carcinoma: comparison with non-neoplastic cholangiole, interlobular duct and septal duct.
Morphometric and immunohistochemical study of cholangiolocellular carcinoma: comparison with non-neoplastic cholangiole, interlobular duct and septal duct.
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DOI：
10.1007/s00534-011-0483-5
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发表时间：
2012-05
影响因子：
3
通讯作者：
Asano, Takehide
中科院分区：
文献类型：
--
作者：
Maeno, Sawako;Kondo, Fukuo;Sano, Keiji;Takada, Tadahiro;Asano, Takehide
胆管细胞癌(CoCC)的起源仍存在争议。为了解决这一问题，对CoCC的形态和免疫组织化学特征进行了研究。癌性导管：13例切除的15个癌前病变和2例尸检。非肿瘤性导管：8例癌旁组织和12例肝细胞癌的非癌区标本。从这些标本中随机选择胆管、小叶间导管(ILD-S)、中型小叶间导管(ILD-M)和间隔导管。测量了这些导管的外径和内径。免疫组织化学：两种肝细胞标记物[HEP-PAR-1和α-胎光蛋白]、两种胆管细胞标记物(细胞角蛋白CK7、CK19)、粘蛋白标记物(MUc1)、肝干/祖细胞标记物(c-Kit)和上皮膜抗原。形态计量学：COCC的外径和内径均值均远大于胆管，介于ILD-S和ILD-M之间。免疫组织化学：所有胆囊癌导管两种肝细胞标志物均为阴性，CK7均阳性。大多数胆囊癌导管CK19阳性。胆管c-Kit的阳性率与胆囊癌最远。胆囊癌、胆管结石和ILD-S中EMA在胆管膜区的阳性表达率同样很高。这些结果表明，CoCCs可能起源于ILDS。
The origin of cholangiolocellular carcinoma (CoCC) is still controversial. To solve this problem, morphometric and immunohistochemical features of CoCC were examined. Cancerous ducts: 15 CoCC lesions from 13 resected and two autopsied cases. Non-neoplastic ducts: 20 specimens of non-cancerous areas of eight resected CoCC cases and of 12 resected hepatocellular carcinoma (HCC) cases. From these specimens, cholangioles, interlobular ducts of small size (ILD-S), interlobular ducts of medium size (ILD-M) and septal ducts were randomly selected. The outer and inner diameters of these ducts were measured. Immunohistochemistry: two hepatocyte markers [Hep Par 1 and α-fetoptotein (AFP)], two cholangiocyte markers (cytokeratin CK7, CK19), a marker for mucin (Muc1), a hepatic stem/progenitor cell marker (c-Kit) and epithelial membrane antigen (EMA) were used. Morphometry: both mean values of the outer and inner diameters of CoCC were far larger than those of cholangioles, and showed intermediate values between those of ILD-S and ILD-M. Immunohistochemistry: all ducts of CoCCs were negative for the two hepatocyte markers and positive for CK 7. Most CoCC ducts were positive for CK 19. Positive rate of c-Kit of cholangiole was most remote from that of CoCC. The positive rates of EMA in the membranous area of ducts were similarly very high in CoCC, cholangiole and ILD-S. These results suggest that CoCCs may originate from ILDs.
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影响因子：
1.3
作者：
Motosugi, Utaroh;Ichikawa, Tomoaki;Yamaguchi, Hiroshi
通讯作者：
Yamaguchi, Hiroshi
DOI：
10.1007/bf00716991
发表时间：
1988-01-01
期刊：
VIRCHOWS ARCHIV A-PATHOLOGICAL ANATOMY AND HISTOPATHOLOGY
影响因子：
--
作者：
KONDO, F;WADA, K;KONDO, Y
通讯作者：
KONDO, Y
影响因子：
6.4
作者：
Kozaka, K.;Sasaki, M.;Nakanuma, Y.
通讯作者：
Nakanuma, Y.
影响因子：
3.3
作者：
Asayama, Yoshiki;Tajima, Tsuyoshi;Honda, Hiroshi
通讯作者：
Honda, Hiroshi
影响因子：
1.3
作者：
Fukukura, Y;Hamanoue, M;Nakajo, M
通讯作者：
Nakajo, M