Sequential multimodal microscopic imaging and biaxial mechanical testing of living multicomponent tissue constructs.
Sequential multimodal microscopic imaging and biaxial mechanical testing of living multicomponent tissue constructs.
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DOI：
10.1007/s10439-014-1019-3
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发表时间：
2014-09
影响因子：
3.8
通讯作者：
Yeh AT
中科院分区：
文献类型：
--
作者：
Bai Y;Lee PF;Humphrey JD;Yeh AT
了解机械刺激和细胞反应之间的关系需要测量不断变化的组织结构和机械性能。我们开发了一种3D组织生物反应器，该生物反应器耦合到定制的多模态显微镜系统的载物台和双轴机械测试平台。成纤维细胞接种的十字形纤维蛋白凝胶的微观结构和力学性能的时间依赖性变化进行了研究，而无论是锚定（1.0：1.0拉伸比）或带双轴（1.0：1.1）条件下培养。一个多模态非线性光学显微镜光学相干显微镜（NLOM-OCM）系统被用来描绘非侵入性的相对空间分布的原始纤维蛋白，沉积的胶原蛋白，成纤维细胞在一个月的培养。还进行了系列培养物内机械试验，以跟踪无菌条件下散装机械性能的演变。在一个月的时间过程中，接种的细胞和沉积的胶原蛋白随机分布在等双轴锚定的结构，但表现出优先排列平行于10%的拉伸下培养的结构条双轴拉伸的方向。令人惊讶的是，锚定和条带双轴拉伸结构都表现出各向同性的机械性能（包括逐渐增加的刚度），尽管开发了非常不同的胶原微结构组织。总之，我们的双轴生物反应器系统集成NLOM-OCM和机械测试提供了关于微观结构组织和机械性能的补充信息，因此，可以更好地理解工程软组织力学和机械生物学之间的关系。
Understanding relationships between mechanical stimuli and cellular responses require measurements of evolving tissue structure and mechanical properties. We developed a 3D tissue bioreactor that couples to both the stage of a custom multimodal microscopy system and a biaxial mechanical testing platform. Time dependent changes in microstructure and mechanical properties of fibroblast seeded cruciform fibrin gels were investigated while cultured under either anchored (1.0:1.0 stretch ratio) or strip biaxial (1.0:1.1) conditions. A multimodal nonlinear optical microscopy-optical coherence microscopy (NLOM-OCM) system was used to delineate noninvasively the relative spatial distributions of original fibrin, deposited collagen, and fibroblasts during month long culture. Serial in-culture mechanical testing was also performed to track the evolution of bulk mechanical properties under sterile conditions. Over the month long time course, seeded cells and deposited collagen were randomly distributed in equibiaxially anchored constructs, but exhibited preferential alignment parallel to the direction of the 10% stretch in constructs cultured under strip biaxial stretch. Surprisingly, both anchored and strip biaxial stretched constructs exhibited isotropic mechanical properties (including progressively increasing stiffness) despite developing a very different collagen microstructural organization. In summary, our biaxial bioreactor system integrating both NLOM-OCM and mechanical testing provided complementary information on microstructural organization and mechanical properties and, thus, may enable greater fundamental understanding of relationships between engineered soft tissue mechanics and mechanobiology.
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DOI：
10.1115/1.1318906
发表时间：
2000-12-01
影响因子：
1.7
作者：
Butler, DL;Goldstein, SA;Guilak, F
通讯作者：
Guilak, F
影响因子：
4.8
作者：
Kume, Teruyoshi;Akasaka, Takashi;Yoshida, Kiyoshi
通讯作者：
Yoshida, Kiyoshi
影响因子：
2.3
作者：
Hartmann, A;Boukamp, P;Friedl, P
通讯作者：
Friedl, P
DOI：
10.1073/pnas.0907813106
发表时间：
2010-02-23
影响因子：
11.1
作者：
Niklason, Laura E.;Yeh, Alvin T.;Humphrey, Jay D.
通讯作者：
Humphrey, Jay D.
影响因子：
3.5
作者：
Humphrey, J. D.;Wells, P. B.;Yeh, A. T.
通讯作者：
Yeh, A. T.