Fusobacteria modulate oral carcinogenesis and promote cancer progression.
Fusobacteria modulate oral carcinogenesis and promote cancer progression.
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梭菌调节口腔癌发生并促进癌症进展。
DOI：
10.1080/20002297.2020.1849493
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发表时间：
2020-11-30
影响因子：
4.5
通讯作者：
Chan EKL
中科院分区：
文献类型：
--
作者：
Harrandah AM;Chukkapalli SS;Bhattacharyya I;Progulske-Fox A;Chan EKL
背景：有证据表明牙周细菌感染可促进口腔癌的发生和发展。目的：利用细胞系统和小鼠致癌模型研究牙周细菌对口腔癌细胞行为的影响。方法：用4种牙周细菌混合感染口腔癌细胞。检测感染后细胞因子水平及癌基因mRNA表达的相对变化。用4-硝基喹啉-1-氧化物(4NQO)诱发小鼠口腔肿瘤，比较了给药前后小鼠口腔肿瘤的变化。结果：多重感染的口腔癌细胞MMP1、MMP9和IL-8表达上调。细胞存活标志物MYC、JAK1、STAT3及上皮-间充质转化标志物ZEB1、转化生长因子β的表达也显著升高。单一感染表明，核杆菌单独的作用与四种细菌的作用相当或更大。梭菌培养上清，主要是脂多糖，足以诱导IL-8的分泌，表明活的梭菌可能不需要与癌细胞直接接触就能改变癌细胞的行为。在4NQO诱导的口腔肿瘤模型中，感染细菌的小鼠与未感染的小鼠相比，出现了明显更大和更多的病变。结论：本研究表明，梭杆菌在口腔肿瘤微环境中存在时，可能会增强癌细胞的侵袭性、存活率和EMT。缩写：4NQO，4-硝基喹啉-1-氧化物；ELISA，酶联免疫吸附试验；EMT，上皮-间质转化；IL-8，白介素8；JAK1，Janus kinase1；脂多糖；MMPs，基质金属蛋白酶；口腔鳞癌；PK，蛋白酶K；PMB，多粘菌素B；qRT-PCR法，实时定量聚合酶链式反应；STAT3，信号转导和转录激活因子3；转化生长因子β，转化生长因子β；ZEB1，锌指E盒结合同源盒1。
Background: Evidence suggest periodontal bacterial infection can contribute to oral cancer initiation and progression. Aim: To investigate the effects of periodontal bacteria on oral cancer cell behavior using a cell-based system and a mouse carcinogenesis model. Methods: Oral cancer cell lines were polyinfected with four periodontal bacteria. Cytokine levels and relative changes in oncogene mRNA expression were determined post-infection. Oral tumours in mice induced by 4-nitroquinoline-1-oxide (4NQO) were compared with and without administrating periodontal bacteria. Results: Polyinfected oral cancer cells had upregulated MMP1, MMP9, and IL-8. The expression of cell survival markers MYC, JAK1, and STAT3 and epithelial-mesenchymal transition markers ZEB1 and TGF-β were also significantly elevated. Monoinfections showed F. nucleatum alone had comparable or greater effects than the four bacteria together. Fusobacterial culture supernatant, primarily LPS, was sufficient to induce IL-8 secretion, demonstrating that direct contact of live Fusobacteria with cancer cells might not be required to exert changes in cancer cell behaviour. In the 4NQO-induced oral tumour model, mice infected with bacteria developed significantly larger and more numerous lesions compared to those not infected. Conclusion: This study demonstrated that Fusobacteria could potentially enhance cancer cell invasiveness, survival, and EMT when presented in the oral tumour microenvironment. Abbreviations: 4NQO, 4-nitroquinoline-1-oxide; ELISA, enzyme-linked immunosorbent assay; EMT, epithelial–mesenchymal transition; IL-8, interleukin-8; JAK1, Janus kinase 1; LPS, lipopolysaccharide; MMP, matrix metalloproteinase; OSCCs, oral squamous cell carcinomas; PK, proteinase K; PMB, Polymyxin B; qRT-PCR, quantitative real-time polymerase chain reaction; STAT3, signal transducer and activator of transcription 3; TGF-β, transforming growth factor beta; ZEB1, zinc finger E-Box binding homeobox 1
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影响因子：
5.4
作者：
Han YW
通讯作者：
Han YW
影响因子：
--
作者：
Binder Gallimidi A;Fischman S;Revach B;Bulvik R;Maliutina A;Rubinstein AM;Nussbaum G;Elkin M
通讯作者：
Elkin M
DOI：
10.1002/hed.2880160506
发表时间：
1994-09-01
影响因子：
2.9
作者：
HAWKINS, BL;HENIFORD, BW;HENDLER, FJ
通讯作者：
HENDLER, FJ
影响因子：
7
作者：
Castellarin, Mauro;Warren, Rene L.;Holt, Robert A.
通讯作者：
Holt, Robert A.
影响因子：
4.8
作者：
Bremmer, Jantine F.;Brakenhoff, Ruud H.;Braakhuis, Boudewijn J. M.
通讯作者：
Braakhuis, Boudewijn J. M.