Fusion-expressed CTB improves both systemic and mucosal T-cell responses elicited by an intranasal DNA priming/intramuscular recombinant vaccinia boosting regimen.
Fusion-expressed CTB improves both systemic and mucosal T-cell responses elicited by an intranasal DNA priming/intramuscular recombinant vaccinia boosting regimen.
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融合表达的 CTB 可改善鼻内 DNA 引发/肌内重组牛痘加强方案引起的全身和粘膜 T 细胞反应。
DOI：
10.1155/2014/308732
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发表时间：
2014
影响因子：
4.1
通讯作者：
Xu J
中科院分区：
文献类型：
--
作者：
Qiu S;Ren X;Ben Y;Ren Y;Wang J;Zhang X;Wan Y;Xu J
以往的研究表明，CTB(霍乱毒素B亚单位)可以作为一种遗传佐剂来增强全身免疫反应。为了进一步研究它是否也可以作为一种遗传佐剂来提高黏膜免疫应答，我们构建了表达OVA-CTB融合抗原的DNA疫苗和重组天坛痘苗(RTTV)。用DNA鼻腔免疫/肌肉注射RTTV方案免疫雌性C57BL/6小鼠。用干扰素-γELISPOT法检测卵子特异性T细胞应答，用ELISA法测定特异性抗体应答。与非佐剂组(PSV-OVA鼻腔免疫/RTTV-OVA肌肉增强)相比，PSV-OVA-CTB鼻腔免疫/RTTV-OVA-CTB肌肉增强组显著提高了脾(1562±567 SFCs/106对330±182 SFCs/106脾细胞，P<；0.01)，肠系膜LN(96±83 SFCs/106对1±2 SFCs/106淋巴细胞，P<；0.05)，呼吸道引流线(109±60 SFCs/106对2±2 SFCs/106淋巴细胞，P<；0.01)。女性生殖道(89±48 SFCs/106淋巴细胞比23±21 SFCs/106淋巴细胞，P<；0.01)。这些结果共同证明，融合表达的CTB可以作为一种有效的佐剂来改善全身和粘膜T细胞的反应。
Previous study showed that CTB (Cholera toxin subunit B) can be used as a genetic adjuvant to enhance the systemic immune responses. To further investigate whether it can also be used as a genetic adjuvant to improve mucosal immune responses, we constructed DNA and recombinant Tiantan vaccinia (rTTV) vaccines expressing OVA-CTB fusion antigen. Female C57BL/6 mice were immunized with an intranasal DNA priming/intramuscular rTTV boosting regimen. OVA specific T-cell responses were measured by IFN-γ ELISPOT and specific antibody responses were determined by ELISA. Compared to the nonadjuvant group (pSV-OVA intranasal priming/rTTV-OVA intramuscular boosting), pSV-OVA-CTB intranasal priming/rTTV-OVA-CTB intramuscular boosting group significantly improved the magnitudes of T-cell responses at spleen (1562 ± 567 SFCs/106 splenocytes versus 330 ± 182 SFCs/106 splenocytes, P < 0.01), mesenteric LN (96 ± 83 SFCs/106 lymphocytes versus 1 ± 2 SFCs/106 lymphocytes, P < 0.05), draining LNs of respiratory tract (109 ± 60 SFCs/106 lymphocytes versus 2 ± 2 SFCs/106 lymphocytes, P < 0.01) and female genital tract (89 ± 48 SFCs/106 lymphocytes versus 23 ± 21 SFCs/106 lymphocytes, P < 0.01). These results collectively demonstrated that fusion-expressed CTB could act as a potent adjuvant to improve both systemic and mucosal T-cell responses.
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影响因子：
3.7
作者：
WILSON, AD;CLARKE, CJ;STOKES, CR
通讯作者：
STOKES, CR
影响因子：
5.5
作者：
Huang, Xianggang;Xu, Jianqing;Shao, Yiming
通讯作者：
Shao, Yiming
影响因子：
--
作者：
Bagley, Kenneth C.;Lewis, George K.;Fouts, Timothy R.
通讯作者：
Fouts, Timothy R.
编码SAG1/SAG3与霍乱毒素A2/B亚基的复合DNA疫苗作为遗传佐剂保护BALB/c小鼠免受弓形虫感染
影响因子：
3.2
作者：
Cong H;Zhang M;Xin Q;Wang Z;Li Y;Zhao Q;Zhou H;He S
通讯作者：
He S