Histomorphometric lung density evaluation of Immulina treatment using a murine influenza pneumonia model.
Histomorphometric lung density evaluation of Immulina treatment using a murine influenza pneumonia model.
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DOI：
10.1111/iep.12493
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发表时间：
2023-12
影响因子：
3
通讯作者：
中科院分区：
文献类型：
--
作者：
采用组织形态计量学肺密度测量来评价Immulina对小鼠肺炎的影响。小鼠鼻内暴露于H1N1流感病毒，剂量为5 × 104 PFU/50 μL/小鼠。使用NIH ImageJ软件程序测量肺密度。将密度值与半定量肺炎严重程度评分进行比较。在25倍、40倍和400倍放大倍率下评价肺显微照片。该研究包括病毒接种对照（IC）和未接种对照（NC）以及接受或未接受Immulina治疗的小鼠。包括三种剂量的Immulina（25、50或100 mg/kg），并使用3种方案施用：预防性治疗（P）、前驱治疗（PD）和治疗性治疗（TH）（注意，在大多数评估中，三种治疗方案的数据是组合的）。在暴露后第3、5、7、10和15天评价小鼠组。“指状肺炎”（DP）的发生定义为密度测量值高于相应NC值的95%置信限。与IC相比，在较高剂量下Immulina治疗的DP发生率的显著降低早在第3天就观察到，并持续至第15天。Immulina还导致肺密度出现统计学显著性剂量变量降低。该研究表明，早期使用Immulina（P或PD方案）可以增强对流感诱导的病毒性肺炎的抵抗力。在25 ×和40 ×图像中观察到肺炎严重程度评分与肺密度之间存在中度相关性（R分别为0.56和0.53），在400 ×图像中观察到强相关性（R = 0.68）。
Histomorphometric lung density measurements were used to evaluate the effects of Immulina on mouse pneumonia. Mice were intra‐nasally exposed to H1N1 influenza virus at a dose of 5 × 104 PFU/50 μL/mouse. Lung density was measured using the NIH ImageJ software program. Density values were compared to semiquantitative pneumonia severity scores. Lung photomicrographs were evaluated at 25‐×, 40‐× and 400‐× magnification. The study included viral inoculated controls (IC) and non‐inoculated controls (NC) and mice either treated or not treated with Immulina. Three doses of Immulina were included (25, 50 or 100 mg/kg) and administered using 3 protocols: prophylactic treatment (P), prodromal treatment (PD) and therapeutic treatment (TH) (note that in most of the evaluations of the data for the three treatment protocols were combined). Groups of mice were evaluated on days 3, 5, 7, 10 and 15 following exposure. The occurrence of “digital pneumonia” (DP) was defined as a density measurement above the 95% confidence limit of the corresponding NC values. A significant reduction in the occurrence of DP with Immulina treatment at the higher doses compared to IC was seen as early as day 3 and persisted up to day 15. There were also statistically significant dose‐variable reductions in lung density in response to Immulina. The study suggests early administration of Immulina (P or PD protocols) may enhance resistance against influenza‐induced viral pneumonia. A moderate correlation between pneumonia severity scores and lung density was observed for the 25‐× and 40‐× images (R = 0.56 and 0.53 respectively), and a strong correlation (R = 0.68) for 400‐× images.
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