Spontaneously established syntrophic yeast communities improve bioproduction.
Spontaneously established syntrophic yeast communities improve bioproduction.
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DOI：
10.1038/s41589-023-01341-2
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发表时间：
2023-08
影响因子：
14.8
通讯作者：
Ledesma-Amaro, Rodrigo
中科院分区：
文献类型：
--
作者：
Aulakh, Simran Kaur;Vidal, Lara Selles;South, Eric J.;Peng, Huadong;Varma, Sreejith Jayasree;Herrera-Dominguez, Lucia;Ralser, Markus;Ledesma-Amaro, Rodrigo
营养相互依赖（syntrophy）有未充分开发的潜力，以改善生物技术过程中使用合作的细胞类型。到目前为止，酵母互养群落的设计需要广泛的遗传操作，因为大多数真核微生物营养缺陷型的共接种不会导致合作生长。在这里，我们采用高通量表型筛选系统测试营养缺陷型酿酒酵母缺失突变体的成对组合。虽然大多数共培养对不进入互养生长，我们确定了49对自发形成互养，协同社区。我们的特点是9个共培养物的稳定性和生长动力学，并证明了一对色氨酸营养缺陷型通过交换途径中间体，而不是最终产品的增长。然后，我们引入了一种在不同营养缺陷型之间分裂的丙二酸半醛生物合成途径，这导致了产量的增加。我们的研究结果报告自发形成稳定的syntrophy在S。酿酒酵母营养缺陷型，并说明了在合作种内社区分工的生物技术潜力。系统地培养营养缺陷型酵母突变体的组合导致鉴定形成强制性交叉喂养关系的对，其中一些随着时间的推移是稳定的，并且可以为生物技术应用划分代谢劳动。
Nutritional codependence (syntrophy) has underexplored potential to improve biotechnological processes by using cooperating cell types. So far, design of yeast syntrophic communities has required extensive genetic manipulation, as the co-inoculation of most eukaryotic microbial auxotrophs does not result in cooperative growth. Here we employ high-throughput phenotypic screening to systematically test pairwise combinations of auxotrophic Saccharomyces cerevisiae deletion mutants. Although most coculture pairs do not enter syntrophic growth, we identify 49 pairs that spontaneously form syntrophic, synergistic communities. We characterized the stability and growth dynamics of nine cocultures and demonstrated that a pair of tryptophan auxotrophs grow by exchanging a pathway intermediate rather than end products. We then introduced a malonic semialdehyde biosynthesis pathway split between different pairs of auxotrophs, which resulted in increased production. Our results report the spontaneous formation of stable syntrophy in S. cerevisiae auxotrophs and illustrate the biotechnological potential of dividing labor in a cooperating intraspecies community. Systematically culturing combinations of auxotrophic yeast mutants leads to the identification of pairs that form obligatory cross-feeding relationships, some of which are stable over time and can divide metabolic labor for biotechnological applications.
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影响因子：
6
作者：
Bernstein HC;Carlson RP
通讯作者：
Carlson RP
影响因子：
4.3
作者：
Germerodt S;Bohl K;Lück A;Pande S;Schröter A;Kaleta C;Schuster S;Kost C
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Kost C
影响因子：
9.3
作者：
Noto Guillen M;Rosener B;Sayin S;Mitchell A
通讯作者：
Mitchell A
影响因子：
5.2
作者：
Jawed, Kamran;Yazdani, Syed Shams;Koffas, Mattheos Ag
通讯作者：
Koffas, Mattheos Ag
影响因子：
8.4
作者：
Lee, Hyewon;Baek, Ji In;Lee, Seung-Goo
通讯作者：
Lee, Seung-Goo