Rice transcription factor OsMADS25 modulates root growth and confers salinity tolerance via the ABA-mediated regulatory pathway and ROS scavenging.
Rice transcription factor OsMADS25 modulates root growth and confers salinity tolerance via the ABA-mediated regulatory pathway and ROS scavenging.
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水稻转录因子 OsMADS25 通过 ABA 介导的调控途径和 ROS 清除调节根系生长并赋予耐盐性
DOI：
10.1371/journal.pgen.1007662
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发表时间：
2018-10
期刊：
影响因子：
4.5
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Xu N;Chu Y;Chen H;Li X;Wu Q;Jin L;Wang G;Huang J
植物根系长期受到各种非生物胁迫的影响，高盐胁迫是限制植物生长的主要条件之一。在这项研究中，我们描述了OsMADS 25是通过维持水稻（Oryza sativa）中ROS稳态来维持根系生长以及耐盐性所必需的。过量表达OsMADS 25基因显著提高了小麦的主根长度和侧根密度，而RNAi沉默OsMADS 25基因则显著降低了主根的伸长，并改变了根尖中活性氧的积累。转录激活实验表明，OsMADS 25通过与其启动子结合直接激活OsGST 4（谷胱甘肽S-转移酶）的表达。同时，OsGST 4突变体表现出生长抑制、对盐和氧化胁迫的高度敏感性，重组OsGST 4蛋白在体外具有清除ROS的活性。结果表明，OsMADS 25基因的过表达显著增强了水稻对盐胁迫和氧化胁迫的耐受性，提高了抗氧化酶活性，增加了植物保护性溶质脯氨酸的积累，降低了气孔开放频率。此外，OsMADS 25通过与其启动子结合而特异性激活脯氨酸生物合成的关键组分OsP 5CR的转录。有趣的是，OsMADS 25的过表达提高了根系对外源阿坝的敏感性，并且在盐胁迫下，过表达植物中依赖于ABA的胁迫响应基因的表达量大幅增加。此外，OsMADS 25似乎通过激活OsYUC 4的转录来促进生长素信号传导。综上所述，OsMADS 25可能是一个重要的转录调控因子，通过ABA介导的调控途径和ROS清除作用调控水稻根系生长，从而赋予水稻耐盐性。植物根系长期受到各种非生物胁迫的影响，高盐是限制植物生长的主要条件之一。在这里，我们表明，转录因子OsMADS 25正调控水稻根系发育和耐盐性和氧化胁迫。我们还提供了强有力的证据表明，OsMADS 25通过直接激活OsGST 4和OsP 5CR的表达来增加ROS清除能力和脯氨酸积累。此外，OsMADS 25促进ABA依赖的非生物胁迫响应调节途径。此外，OsMADS 25似乎通过激活OsYUC 4转录来促进生长素信号传导。总之，通过ABA介导的调节途径增强抗氧化反应和脯氨酸积累被认为是OsMADS 25调节水稻耐盐性的关键。
Plant roots are constantly exposed to a variety of abiotic stresses, and high salinity is one of the major limiting conditions that impose constraints on plant growth. In this study, we describe that OsMADS25 is required for the root growth as well as salinity tolerance, via maintaining ROS homeostasis in rice (Oryza sativa). Overexpression of OsMADS25 remarkably enhanced the primary root (PR) length and lateral root (LR) density, whereas RNAi silence of this gene reduced PR elongation significantly, with altered ROS accumulation in the root tip. Transcriptional activation assays indicated that OsMADS25 activates OsGST4 (glutathione S–transferase) expression directly by binding to its promoter. Meanwhile, osgst4 mutant exhibited repressed growth and high sensitivity to salinity and oxidative stress, and recombinant OsGST4 protein was found to have ROS–scavenging activity in vitro. Expectedly, overexpression of OsMADS25 significantly enhanced the tolerance to salinity and oxidative stress in rice plants, with the elevated activity of antioxidant enzymes, increased accumulation of osmoprotective solute proline and reduced frequency of open stoma. Furthermore, OsMADS25 specifically activated the transcription of OsP5CR, a key component of proline biosynthesis, by binding to its promoter. Interestingly, overexpression of OsMADS25 raised the root sensitivity to exogenous ABA, and the expression of ABA–dependent stress–responsive genes was elevated greatly in overexpression plants under salinity stress. In addition, OsMADS25 seemed to promote auxin signaling by activating OsYUC4 transcription. Taken together, our findings reveal that OsMADS25 might be an important transcriptional regulator that regulates the root growth and confers salinity tolerance in rice via the ABA–mediated regulatory pathway and ROS scavenging. Plant roots are constantly exposed to a variety of abiotic stresses, and high salinity is one of major limiting conditions that impose constraints on plant growth. Here, we show that transcription factor OsMADS25 positively regulates the root system development and tolerance to salinity and oxidative stress in rice plants. We also provide strong evidence that OsMADS25 increases the ROS-scavenging capacity and proline accumulation by activating the expression of OsGST4 and OsP5CR directly. Moreover, OsMADS25 promotes ABA–dependent abiotic stress–responsive regulatory pathway. In addition, OsMADS25 seems to promote auxin signaling by activating OsYUC4 transcription. Overall, enhanced antioxidant responses and proline accumulation via the ABA–mediated regulatory pathway, have been proposed to be crucial for OsMADS25 to regulate the salinity tolerance in rice plants.
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