The Effect of Early Maternal Separation Combined With Adolescent Chronic Unpredictable Mild Stress on Behavior and Synaptic Plasticity in Adult Female Rats.
The Effect of Early Maternal Separation Combined With Adolescent Chronic Unpredictable Mild Stress on Behavior and Synaptic Plasticity in Adult Female Rats.
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早期母亲分离结合青春期慢性不可预测的轻度应激对成年雌性大鼠行为和突触可塑性的影响
DOI：
10.3389/fpsyt.2021.539299
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发表时间：
2021
影响因子：
4.7
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Huang J;Shen C;Ye R;Shi Y;Li W
本研究旨在评价早期母源分离（MS）联合青春期慢性不可预知轻度应激（CIMs）建立的雌性成年SD大鼠抑郁模型，观察大鼠行为学及突触蛋白表达，为抗抑郁药物活性筛选提供参考。本研究采用MS和TNS建立雌性大鼠双重应激模型。行为学测试包括蔗糖偏好实验、旷场实验和零迷宫实验，用于检测动物的抑郁样和焦虑样行为。采用尼氏染色法检测各组大鼠海马CA 1区和DG区神经细胞数。Western blot检测海马突触素（SYN）、突触后密度-95（PSD-95）和生长相关蛋白-43（GAP-43）的表达。免疫组化法检测海马SYN蛋白的表达。与MS组相比，MS+ BNS组大鼠表现出更严重的抑郁样和焦虑样行为。海马CA 1区和DG区尼氏体减少，SYN阳性细胞减少，SYN、PSD-95和GAP 43表达下调。结论：MS和TNS组成年雌性大鼠表现出更多的抑郁样和焦虑样行为，这一过程可能与突触可塑性受损有关。
Our aims were to evaluate the depression model of early maternal separation (MS) combined with adolescent chronic unpredictable mild stress (CUMS) in female adult SD rats to observe the behavior and the expressions of synaptic proteins in rats and to provide a reference for the screening of antidepressant drug activity. In our study, MS and CUMS were conducted to establish a dual stress model on female rats. Behavioral tests, including the sucrose preference test, open field test, and zero maze test, were used to detect depression-like and anxiety-like behavior of animals. Nissl staining was used to detect the number of neuronal cells in the hippocampus CA1 and DG regions of rats from each group. Synaptophysin (SYN), postsynaptic density-95 (PSD-95), and growth-associated protein-43 (GAP-43) expressions in the hippocampus were detected by western blot. Expression of the hippocampus SYN protein was further detected by immunohistochemistry. Rats in the MS+CUMS group presented more serious depression-like and anxiety-like behavior than in the MS group. Also, few Nissl bodies in the hippocampus CA1 and DG regions, less percentage of SYN-positive cells, and downregulated expressions of SYN, PSD-95, and GAP43 were found in the hippocampus of rats in MS+CUMS group. In conclusion, adult female rats that underwent MS and CUMS performed more critical depression-like and anxiety-like behaviors, and this process may be resulted from synaptic plasticity impairment.
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影响因子：
7.4
作者：
Garber, Judy;Brunwasser, Steven M.;Zerr, Argero A.;Schwartz, Karen T. G.;Sova, Karen;Weersing, V. Robin
通讯作者：
Weersing, V. Robin
抑郁症大鼠模型中嗅觉受体神经元数量减少
影响因子：
2.5
作者：
Li, Qianlu;Yang, Deyu;Li, Zhiwei
通讯作者：
Li, Zhiwei
影响因子：
64.3
作者：
Kuehner, Christine
通讯作者：
Kuehner, Christine
DOI：
10.1016/j.pnpbp.2015.09.003
发表时间：
2016-02-04
影响因子：
5.6
作者：
Gracia-Rubio, Irene;Moscoso-Castro, Maria;Valverde, Olga
通讯作者：
Valverde, Olga
影响因子：
82.9
作者：
Kang, Hyo Jung;Voleti, Bhavya;Hajszan, Tibor;Rajkowska, Grazyna;Stockmeier, Craig A.;Licznerski, Pawel;Lepack, Ashley;Majik, Mahesh S.;Jeong, Lak Shin;Banasr, Mounira;Son, Hyeon;Duman, Ronald S.
通讯作者：
Duman, Ronald S.