Transcriptomic and Metabolomic Studies Disclose Key Metabolism Pathways Contributing to Well-maintained Photosynthesis under the Drought and the Consequent Drought-Tolerance in Rice.
Transcriptomic and Metabolomic Studies Disclose Key Metabolism Pathways Contributing to Well-maintained Photosynthesis under the Drought and the Consequent Drought-Tolerance in Rice.
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转录组学和代谢组学研究揭示了干旱条件下维持光合作用的关键代谢途径以及水稻的抗旱性。
DOI：
10.3389/fpls.2016.01886
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发表时间：
2016
影响因子：
5.6
通讯作者：
Luo L
中科院分区：
文献类型：
--
作者：
Ma X;Xia H;Liu Y;Wei H;Zheng X;Song C;Chen L;Liu H;Luo L
与野生物种相比，作物的耐旱性需要在干旱期间进行充分的功能性代谢（特别是光合作用过程）。然而，耐旱性，干旱期间的光合调节，以及相关的转录和代谢基础之间的联系，在很大程度上仍然未知。在这项研究中，我们使用了两个水稻品种与对比耐旱性（耐旱品种IRAT 109和耐旱品种IAC 1246），以探讨转录和代谢的长期干旱的反应。在土壤层较浅的改良田中，耐旱品种具有较高的渗透调节能力和抗氧化能力，在渐进性干旱胁迫下表现出较高的相对光合速率。在干旱和充分供水条件下，分别在IRAT 109和IAC 1246中鉴定了4059和2677个差异表达基因（DEG）。在IRAT 109和IAC 1246中，两种处理之间分别鉴定出共计69种和47种差异代谢物（DM）。与IRAT 109相比，IAC 1246的DEG在干旱期间表现出增强的调节幅度。我们发现DEGs和渗透压和总抗氧化能力（AOC）的两个品种之间的显着相关性。在干旱初期，我们检测到与光合作用相关的DEGs在IAC 1246中上调，这与其较高的相对光合速率相一致。6种差异代谢产物的含量与渗透势和AOC相关。此外，它们在两个品种之间的调节也不同。特别是4-羟基肉桂酸和阿魏酸的上调与干旱早期IAC 1246光合相关DEGs的表现一致。因此，4-羟基肉桂酸和阿魏酸被认为是水稻抗旱性的关键代谢产物。参与这些代谢途径的DEG有望成为提高植物耐旱性的候选基因。总之，干旱条件下维持良好的光合作用有助于提高水稻的耐旱性。代谢产物通过渗透调节和/或抗氧化机制在保护脱水下的光合作用中起重要作用。因此，基于代谢产物的方法是寻找干旱候选基因的有效途径。对干旱胁迫下的代谢伴随转录响应的研究有待进一步深入，以寻找更多有用的代谢产物、途径和基因。
In contrast to wild species, drought-tolerance in crops requires a fully functional metabolism during drought (particularly photosynthetic processes). However, the link between drought-tolerance, photosynthetic regulation during drought, and the associated transcript and metabolic foundation, remains largely unknown. For this study, we used two rice cultivars with contrasting drought-tolerance (the drought-intolerant cultivar IRAT109 and the drought-tolerant cultivar IAC1246) to explore transcript and metabolic responses to long-term drought. The drought-tolerant cultivar represented higher osmotic adjustment and antioxidant capacity, as well as higher relative photosynthesis rate under a progressive drought stress occurred in a modified field with shallow soil-layers. A total of 4059 and 2677 differentially expressed genes (DEGs) were identified in IRAT109 and IAC1246 between the drought and well-watered conditions, respectively. A total of 69 and 47 differential metabolites (DMs) were identified between the two treatments in IRAT109 and IAC1246, respectively. Compared to IRAT109, the DEGs of IAC1246 displayed enhanced regulatory amplitude during drought. We found significant correlations between DEGs and the osmolality and total antioxidant capacity (AOC) of both cultivars. During the early stages of drought, we detected up-regulation of DEGs in IAC1246 related to photosynthesis, in accordance with its higher relative photosynthesis rate. The contents of six differential metabolites were correlated with the osmotic potential and AOC. Moreover, they were differently regulated between the two cultivars. Particularly, up-regulations of 4-hydroxycinnamic acid and ferulic acid were consistent with the performance of photosynthesis-related DEGs at the early stages of drought in IAC1246. Therefore, 4-hydroxycinnamic acid and ferulic acid were considered as key metabolites for rice drought-tolerance. DEGs involved in pathways of these metabolites are expected to be good candidate genes to improve drought-tolerance. In conclusion, well-maintained photosynthesis under drought should contribute to improved drought-tolerance in rice. Metabolites play vital roles in protecting photosynthesis under dehydration via osmotic adjustments and/or antioxidant mechanisms. A metabolite-based method was thus an effective way to explore drought candidate genes. Metabolic accompanied by transcript responses to drought stress should be further studied to find more useful metabolites, pathways, and genes.
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影响因子：
7.3
作者：
Kotchoni, Simeon O.;Kuhns, Christine;Bartels, Dorothea
通讯作者：
Bartels, Dorothea
影响因子：
5.2
作者：
Prince, Silvas J.;Joshi, Trupti;Nguyen, Henry T.
通讯作者：
Nguyen, Henry T.
影响因子：
7.2
作者：
Saijo, Y;Hata, S;Izui, K
通讯作者：
Izui, K
影响因子：
7.3
作者：
Parent, Boris;Suard, Benoit;Tardieu, Francois
通讯作者：
Tardieu, Francois
影响因子：
5.2
作者：
Jung, Harin;Lee, Dong-Keun;Kim, Ju-Kon
通讯作者：
Kim, Ju-Kon