Exogenous DA-6 Improves the Low Night Temperature Tolerance of Tomato Through Regulating Cytokinin.

Exogenous DA-6 Improves the Low Night Temperature Tolerance of Tomato Through Regulating Cytokinin.
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外源DA-6通过调节细胞分裂素提高番茄夜间低温耐受性

DOI:
10.3389/fpls.2020.599111
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发表时间:
2020
影响因子:
5.6
通讯作者:
Liu Y
Liu Y
中科院分区:
生物学2区
文献类型:
--
作者:
Lu J;Guan P;Gu J;Yang X;Wang F;Qi M;Li T;Liu Y

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夜间低温(LNT)会引起环境胁迫,并对植物生长和生产力产生严重的负面影响。人工合成的诱导子可以调节植物的生长并诱导植物对这种胁迫的防御机制。在这里,我们评估了外源生长调节剂二乙基氨基乙基己酸(DA-6)在番茄叶片响应LNT胁迫的影响。结果表明,外源DA-6激活了叶绿素合成和光系统相关基因的表达,导致较高的光合活性和叶绿素产量。此外,DA-6还可以调节内源细胞分裂素(CTK)的合成和降解基因的表达,从而稳定叶绿体结构,减轻氧化损伤,维持LNT胁迫下番茄叶片的光化学活性。DA-6维持较高水平的阿坝含量并诱导CBF基因的表达,表明DA-6可能参与了冷响应信号通路,诱导下游低温响应基因的表达和相容性渗透调节物质的积累。本研究揭示了植物生长调节剂提高植物耐低温性的作用机制,为植物生长调节剂缓解低温胁迫的不利影响提供了重要的参考依据。
Low night temperature (LNT) causes environmental stress and has a severe and negative impact on plant growth and productivity. Synthetic elicitors can regulate plant growth and induce defense mechanisms from this type of stress. Here, we evaluated the effect of the exogenous growth regulator diethyl aminoethyl hexanoate (DA-6) in tomato leaf response to LNT stress. Our results showed that exogenous DA-6 activates the expression of chlorophyll synthesis and photosystem-related genes, and results in higher photosynthetic activity and chlorophyll production. Furthermore, DA-6 can regulate the synthesis of endogenous cytokinin (CTK) and the expression of decomposition genes to stabilize chloroplast structure, reduce oxidative damage, and maintain the photochemical activity of tomato leaves under LNT stress. DA-6 maintains a high level of ABA content and induces the expression of CBF genes, indicating that DA-6 may participate in the cold response signaling pathway and induce the expression of downstream low temperature response genes and accumulation of compatible osmolytes. This study unravels a mode of action by which plant growth regulators can improve low temperature tolerance and provides important considerations for their application to alleviate the harmful effects of cold stress.
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