Presence and Characterization of a Novel cfr-Carrying Tn558 Transposon Derivative in Staphylococcus delphini Isolated From Retail Food.
Presence and Characterization of a Novel cfr-Carrying Tn558 Transposon Derivative in Staphylococcus delphini Isolated From Retail Food.
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从零售食品中分离的飞燕草葡萄球菌中携带 Tn558 转座子衍生物的新型 cfr 的存在和表征
DOI：
10.3389/fmicb.2020.598990
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发表时间：
2020
影响因子：
5.2
通讯作者：
Wu Q
中科院分区：
文献类型：
--
作者：
Zhang F;Wu S;Huang J;Yang R;Zhang J;Lei T;Dai J;Ding Y;Xue L;Wang J;Chen M;Wu Q
抗菌素抵抗已成为主要的公共卫生威胁。与食品相关的葡萄球菌物种由于多药的耐药性而受到了很多关注。与多药耐药性相关的CFR基因在食品衍生的葡萄球菌物种中一直始终检测到。在这项回顾性研究中，我们研究了2011年至2016年从中国不同地理区域中从家禽肉中分离出的CFR阳性葡萄球菌菌株的普遍性。从中国的家禽肉中鉴定出了两种CFR阳性葡萄球菌Delphini菌株。进行了比较和全基因组分析，以表征两个链球菌分离株245-1和2794-1中的遗传特征和总体抗菌抗性基因。全基因组测序表明，他们俩都藏有一种新型的20,258 bp CFR携带的TN558转座子衍生物。 TN558衍生物具有多种抗菌抗性基因，包括可转移的多抑制基因CFR，氯霉素耐药性基因FEXA，氨基糖苷耐药基因AACA-ACA-ACHD和AADD和AADD和AADD和BLEOMYCIN抗性基因抗性。令人惊讶的是，在TN558衍生物中，通过两步pcr鉴定了一个活跃的非常规的可循环结构，该结构包含各种电阻基因和直接重复序列的副本。此外，核心基因组系统发育分析表明，CFR阳性的Delphini菌株与2017年从日本分离的S. delphini 14S03313-1和从瑞士分离的14S03319-1与瑞士分离的14S03319-1最密切相关，2017年从瑞士分离出来。这一发现引起了人们对食品安全和公共卫生安全的潜在威胁的进一步担忧。来自各种葡萄球菌物种的类似CFR携带转座子的发生和传播需要进一步监视。
Antimicrobial resistance has become a major public health threat. Food-related Staphylococcus species have received much attention due to their multidrug resistance. The cfr gene associated with multidrug resistance has been consistently detected in food-derived Staphylococcus species. In this retrospective study, we examined the prevalence of cfr-positive Staphylococcus strains isolated from poultry meat in different geographical areas of China from 2011 to 2016. Two cfr-positive Staphylococcus delphini strains were identified from poultry meat in China. Comparative and whole-genome analyses were performed to characterize the genetic features and overall antimicrobial resistance genes in the two S. delphini isolates 245-1 and 2794-1. Whole-genome sequencing showed that they both harbored a novel 20,258-bp cfr-carrying Tn558 transposon derivative on their chromosomes. The Tn558 derivative harbors multiple antimicrobial resistance genes, including the transferable multiresistance gene cfr, chloramphenicol resistance gene fexA, aminoglycoside resistance genes aacA-aphD and aadD, and bleomycin resistance gene ble. Surprisingly, within the Tn558 derivative, an active unconventional circularizable structure containing various resistance genes and a copy of a direct repeat sequence was identified by two-step PCR. Furthermore, core genome phylogenetic analysis revealed that the cfr-positive S. delphini strains were most closely related to S. delphini 14S03313-1 isolated from Japan in 2017 and 14S03319-1 isolated from Switzerland in 2017. This study is the first report of S. delphini harboring a novel cfr-carrying Tn558 derivative isolated from retail food. This finding raises further concerns regarding the potential threat to food safety and public health safety. The occurrence and dissemination of similar cfr-carrying transposons from diverse Staphylococcus species need further surveillance.
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影响因子：
3.7
作者：
Azpiroz MF;Bascuas T;Laviña M
通讯作者：
Laviña M
影响因子：
3.4
作者：
Decristophoris, Paola;Fasola, Amy;Petrini, Orlando
通讯作者：
Petrini, Orlando
DOI：
10.1016/j.diagmicrobio.2019.01.014
发表时间：
2019-07-01
影响因子：
2.9
作者：
Magleby, Reed;Bemis, David A.;Westblade, Lars F.
通讯作者：
Westblade, Lars F.
影响因子：
4.9
作者：
Palmieri, Claudio;Mingoia, Marina;Varaldoa, Pietro E.
通讯作者：
Varaldoa, Pietro E.
DOI：
10.1099/ijsem.0.001673
发表时间：
2017-03-01
影响因子：
2.8
作者：
Brawand, Stefanie Gobeli;Cotting, Kerstin;Perreten, Vincent
通讯作者：
Perreten, Vincent