Transcriptomic analyses reveal the adaptive features and biological differences of guts from two invasive whitefly species.
Transcriptomic analyses reveal the adaptive features and biological differences of guts from two invasive whitefly species.
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转录组分析揭示了两种入侵粉虱物种肠道的适应性特征和生物学差异
DOI：
10.1186/1471-2164-15-370
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发表时间：
2014-05-15
期刊：
影响因子：
4.4
通讯作者：
Wang XW
中科院分区：
文献类型：
--
作者：
Ye XD;Su YL;Zhao QY;Xia WQ;Liu SS;Wang XW
韧皮部取食昆虫的肠道对于营养吸收和外源物质的降解是至关重要的。结果利用Illumina测序技术分析了烟粉虱复合体中两种入侵烟粉虱的肠道转录本：中东小亚种1号(MEAM1)和地中海型(MED)。共有12,879个MEAM1转录本和11,246个MED转录本被用显著的Blastx命中注释。此外，MEAM1和MED分别鉴定出7,000和5,771个肠道特异基因。对这些肠道特异基因的功能分析表明，肠道在杀虫剂和次生植物化学物质的代谢中起着重要作用。为了揭示MEAM1和MED肠道的分子差异，对两个物种的肠道转录进行了比较，共鉴定出3910对同源基因。根据非同义替换和同义替换的比率，发现15个基因在正选择下进化。据预测，其中许多基因与新陈代谢和抗药性有关。此外，许多与解毒相关的基因在MED肠道中的表达水平高于MEAM1，这可能是MED对杀虫剂和环境胁迫具有更高抗性的原因。结论MED和MEAM1肠道转录本的测序以及MEAM1和MED肠道转录本的广泛比较为揭示肠道在粉虱中的作用提供了大量的序列信息。
BackgroundThe gut of phloem feeding insects is critical for nutrition uptake and xenobiotics degradation. However, partly due to its tiny size, genomic information for the gut of phloem feeding insects is limited.ResultsIn this study, the gut transcriptomes of two species of invasive whiteflies in theBemisia tabacicomplex, Middle East Asia Minor 1 (MEAM1) and Mediterranean (MED), were analyzed using the Illumina sequencing. A total of 12,879 MEAM1 transcripts and 11,246 MED transcripts were annotated with a significant Blastx hit. In addition, 7,000 and 5,771 gut specific genes were respectively identified for MEAM1 and MED. Functional analyses on these gut specific genes demonstrated the important roles of gut in metabolism of insecticides and secondary plant chemicals. To reveal the molecular difference between guts of MEAM1 and MED, a comparison between gut transcriptomes of the two species was conducted and 3,910 pairs of orthologous genes were identified. Based on the ratio of nonsynonymous and synonymous substitutions, 15 genes were found evolving under positive selection. Many of those genes are predicted to be involved in metabolism and insecticide resistance. Furthermore, many genes related to detoxification were expressed at an elevated level in the gut of MED compared to MEAM1, which might be responsible for the MED’s higher resistance to insecticides and environmental stresses.ConclusionThe sequencing of MED and MEAM1 gut transcriptomes and extensive comparisons of MEAM1 and MED gut transcripts provide substantial sequence information for revealing the role of gut in whiteflies.
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影响因子：
4
作者：
Firdaus, Syarifin;Vosman, Ben;van Heusden, Adriaan Willem
通讯作者：
van Heusden, Adriaan Willem
影响因子：
3.2
作者：
Ghanim, M;Morin, S;Czosnek, H
通讯作者：
Czosnek, H
影响因子：
4.1
作者：
Gorman, Kevin;Slater, Russell;Denholm, Ian
通讯作者：
Denholm, Ian
新发现的烟粉虱共生体的特征（半翅目：粉虱科）
影响因子：
4.4
作者：
Bing, Xiao-Li;Yang, Jiao;Liu, Shu-Sheng
通讯作者：
Liu, Shu-Sheng
交配行为、生活史和对杀虫剂的适应决定了粉虱之间的物种排斥
影响因子：
4.8
作者：
Crowder, David W.;Horowitz, A. Rami;Carriere, Y.
通讯作者：
Carriere, Y.