Probing spatial organization of DNA strands using enzyme-free hairpin assembly circuits.
Probing spatial organization of DNA strands using enzyme-free hairpin assembly circuits.
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DOI：
10.1021/ja300984b
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发表时间：
2012-08-29
影响因子：
15
通讯作者：
Ellington, Andrew D.
中科院分区：
文献类型：
--
作者：
Li, Bingling;Jiang, Yu;Chen, Xi;Ellington, Andrew D.
催化发夹组装(ChA)是一种强大的无酶信号放大反应，具有广泛的潜在应用，特别是在生物传感方面。虽然大多数对CHA分析应用的研究都集中在生物分子浓度的测量上，但我们在这里展示了CHA也可以用来探索单链DNA等生物分子的空间组织。这种检测的基础是这样一个事实，即使立足点和分支迁移域接近的DNA结构可以催化CHA反应。我们定量地研究了这一现象，并将其应用于检测DNA自组装过程中发生的结构域重组，如杂交链式反应(HCR)。我们还展示了CHA电路可以被设计来检测某些类型的杂交缺陷。这一原理使我们能够开发出一种‘信号开启’分析，可以在一个简单的反应中同时对DNA链中的多种类型的突变做出反应，这在基因分型和分子诊断学中非常有意义。这些发现突出了DNA电路对DNA纳米技术的潜在影响，并为这些领域的进一步发展提供了新的工具。
Catalyzed hairpin assembly (CHA) is a robust enzyme-free signal-amplification reaction that has a wide range of potential applications especially in biosensing. Although most studies on the analytical applications of CHA focus on the measurement of concentrations of biomolecules, we show here CHA can also be used to probe the spatial organization of biomolecules such as single-stranded DNA. The basis of such detection is the fact that a DNA structure that brings a toehold and a branch migration domain into close proximity can catalyze the CHA reaction. We quantitatively studied this phenomenon and applied it to the detection of domain reorganization that occurs during DNA self-assembly processes such as hybridization chain reaction (HCR). We also show CHA circuits can be designed to detect certain types of hybridization defects. This principle allowed us to develop a ‘signal-on’ assay that can simultaneously respond to multiple types of mutations in a DNA strand in one simple reaction, which is of great interest in genotyping and molecular diagnostics. These findings highlight the potential impacts of DNA circuitry on DNA nanotechnology and provide new tools for further development of these fields.
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无酶DNA电路对多种检测方法的有理模块化适应。
影响因子：
14.9
作者：
Li B;Ellington AD;Chen X
通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.0407024101
发表时间：
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影响因子：
11.1
作者：
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通讯作者：
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影响因子：
62.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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