Genetic evidence for the vital function of Osterix in cementogenesis.
Genetic evidence for the vital function of Osterix in cementogenesis.
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DOI：
10.1002/jbmr.1552
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发表时间：
2012-05
影响因子：
6.2
通讯作者：
Feng, Jian Q.
中科院分区：
文献类型：
--
作者：
Cao, Zhengguo;Zhang, Hua;Zhou, Xin;Han, Xianglong;Ren, Yinshi;Gao, Tian;Xiao, Yin;de Crombrugghe, Benoit;Somerman, Martha J.;Feng, Jian Q.
到目前为止，再生完整牙齿的尝试，包括与牙根相关的关键牙周组织，都没有成功。关于细胞牙骨质的细胞来源（上皮或间充质）的起源仍然存在争议。这种分歧可能部分是由于缺乏对导致牙根和支持组织（如牙骨质）发生和发育的事件的理解。Osterix（OSX）是骨生成所必需的转录因子，但其在牙骨质形成中的作用尚未得到解决。在本研究中，我们首先记录了OSX的时空表达模式和细胞牙骨质形成之间的密切关系。然后，我们产生了3.6 Col 1-OSX转基因小鼠，其相对于WT对照显示出加速的牙骨质形成。重要的是，在具有两种不同Cre系统（2.3kb Col 1和诱导型CAG CreER）的间充质细胞中OSX的条件性缺失导致牙骨质形成（包括牙骨质质量和矿物质沉积速率）和牙本质基质蛋白1（DMP 1）的基因表达的急剧减少。然而，OSX基因的细胞牙骨质形成后的删除并没有改变成熟的牙骨质的性能进行评估，背散射扫描电镜和树脂铸型扫描电镜。Osx基因转染成牙骨质细胞后，可明显抑制成牙骨质细胞的增殖，促进成牙骨质细胞的分化和矿化。总之，这些数据支持1）细胞牙骨质的间充质起源（来自PDL祖细胞）; 2）OSX在控制细胞牙骨质形成中的重要作用;和3）成年小鼠中细胞牙骨质的有限重塑。
To date, attempts to regenerate a complete tooth, including the critical periodontal tissues associated with the tooth root, have not been successful. Controversy still exists regarding the origin of the cell source for cellular cementum (epithelial or mesenchymal). This disagreement may be partially due to a lack of understanding of the events leading to the initiation and development of the tooth roots and supportive tissues, such as the cementum. Osterix (OSX) is a transcriptional factor essential for osteogenesis, but its role in cementogenesis has not been addressed. In the present study, we first documented a close relationship between the temporal-and spatial-expression pattern of OSX and the formation of cellular cementum. We then generated 3.6 Col 1-OSX transgenic mice, which displayed accelerated cementum formation vs. WT controls. Importantly, the conditional deletion of OSX in the mesenchymal cells with two different Cre systems (the 2.3 kb Col 1 and an inducible CAG-CreER) led to a sharp reduction in cellular cementum formation (including the cementum mass and mineral deposition rate) and gene expression of dentin matrix protein 1 (DMP1) by cementocytes. However, the deletion of the OSX gene after cellular cementum formed did not alter the properties of the mature cementum as evaluated by backscattered SEM and resin-cast SEM. Transient transfection of Osx in the cementoblasts in vitro significantly inhibited cell proliferation and increased cell differentiation and mineralization. Taken together, these data support 1) the mesenchymal origin of cellular cementum (from PDL progenitor cells); 2) the vital role of OSX in controlling the formation of cellular cementum; and 3) the limited remodeling of cellular cementum in adult mice.
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影响因子：
2.9
作者：
Braut, A;Kalajzic, I;Mina, M
通讯作者：
Mina, M
影响因子：
4.1
作者：
Ciani, Cesare;Doty, Stephen B.;Fritton, Susannah R.
通讯作者：
Fritton, Susannah R.
DOI：
10.1073/pnas.0504750102
发表时间：
2005-10-11
影响因子：
11.1
作者：
Akiyama, H;Kim, JE;de Crombrugghe, B
通讯作者：
de Crombrugghe, B
影响因子：
0.7
作者：
Liu, F;Woitge, HW;Kream, BE
通讯作者：
Kream, BE
影响因子：
7.6
作者：
Feng, JQ;Huang, H;Mishina, Y
通讯作者：
Mishina, Y