Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics.
Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics.
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DOI：
10.1063/5.0027022
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发表时间：
2020-12
影响因子：
6
通讯作者：
Taylor RE
中科院分区：
文献类型：
--
作者：
Wang W;Arias DS;Deserno M;Ren X;Taylor RE
事实证明，DNA纳米技术非常擅长探测和操纵生物环境，因为它可以创造出几乎任意形状的纳米结构，允许无数种类型的修饰，所有这些都具有内在的生物兼容性。特别感兴趣的新兴领域是涉及细胞膜的应用，但要充分探索可能性的范围，需要DNA纳米技术、细胞和膜生物学以及生物物理学的跨学科知识。在这篇综述中，我们旨在对这三个领域的交集进行简要的介绍。在简要回顾了DNA纳米技术以及脂质双层和细胞膜的生物和机械性质之后，我们总结了介导膜和DNA纳米结构之间相互作用的策略，重点是程序化递送到脂膜、进入脂膜和通过脂膜。我们还重点介绍了新出现的应用，包括膜雕刻、多细胞自组装、配体和蛋白质的空间排列和组织、生物力学传感、合成DNA纳米孔、生物成像和生物电子传感。许多关键但令人兴奋的挑战摆在前面，我们概述了在转化DNA纳米结构用于未来体外和体内膜应用时给我们带来的前景光明的方向。
DNA nanotechnology has proven exceptionally apt at probing and manipulating biological environments as it can create nanostructures of almost arbitrary shape that permit countless types of modifications, all while being inherently biocompatible. Emergent areas of particular interest are applications involving cellular membranes, but to fully explore the range of possibilities requires interdisciplinary knowledge of DNA nanotechnology, cell and membrane biology, and biophysics. In this review, we aim for a concise introduction to the intersection of these three fields. After briefly revisiting DNA nanotechnology, as well as the biological and mechanical properties of lipid bilayers and cellular membranes, we summarize strategies to mediate interactions between membranes and DNA nanostructures, with a focus on programmed delivery onto, into, and through lipid membranes. We also highlight emerging applications, including membrane sculpting, multicell self-assembly, spatial arrangement and organization of ligands and proteins, biomechanical sensing, synthetic DNA nanopores, biological imaging, and biomelecular sensing. Many critical but exciting challenges lie ahead, and we outline what strikes us as promising directions when translating DNA nanostructures for future in vitro and in vivo membrane applications.
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影响因子：
16.6
作者：
Burns, Jonathan R.;Goepfrich, Kerstin;Wood, James W.;Thacker, Vivek V.;Stulz, Eugen;Keyser, Ulrich F.;Howorka, Stefan
通讯作者：
Howorka, Stefan
影响因子：
16.6
作者：
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通讯作者：
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影响因子：
7
作者：
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通讯作者：
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影响因子：
64.8
作者：
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通讯作者：
Hogberg, Bjorn
影响因子：
38.3
作者：
Burns, Jonathan R.;Seifert, Astrid;Howorka, Stefan
通讯作者：
Howorka, Stefan