Importance of integrity of cell-cell junctions for the mechanics of confluent MDCK II cells.
Importance of integrity of cell-cell junctions for the mechanics of confluent MDCK II cells.
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DOI：
10.1038/s41598-018-32421-2
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发表时间：
2018-09-20
影响因子：
4.6
通讯作者：
Janshoff A
中科院分区：
文献类型：
--
作者：
Brückner BR;Janshoff A
细胞间连接是上皮细胞间重要的机械耦合器，提供粘附和细胞间通讯。连接的调节发生在细胞过程中，如层形成、上皮细胞向间质细胞转变、胚胎发生和癌症进展。许多研究解决了细胞中产生力的作用，以建立横向细胞-细胞连接，以及细胞力传递在组织形成和维持中的作用。我们基于原子力显微镜（AFM）的研究揭示了紧密连接和粘附连接在作为融合单层的一部分的单个上皮细胞的机械特性中的作用。我们发现紧密连接对于建立功能性屏障形成层很重要，但损害它们并不会降低细胞的机械完整性。ZO-1的缺失导致皮质张力的微弱增加。使用DTT对e -钙粘蛋白介导的粘附连接的破坏观察到相反的效果。粘附体连接的开放导致细胞力学的实质性改变，如整体刚度的降低，但这些变化在通过去除DTT重建e-钙粘蛋白中的二硫桥后被证明是可逆的。我们发现，在粘附体断裂连接恢复过程中，存在保持机械完整性的调节机制。
Intercellular junctions are important mechanical couplers between cells in epithelial layers providing adhesion and intercellular communication. Regulation of the junctions occurs in cellular processes such as layer formation, epithelial-to-mesenchymal transition, embryogenesis, and cancer progression. Many studies addressed the role of force generation in cells for establishing lateral cell-cell junctions and the role of cellular force transmission in tissue formation and maintenance. Our atomic force microscopy- (AFM) based study shed light on the role of both, tight junctions and adherens junctions for the mechanical properties of individual epithelial cells that are part of a confluent monolayer. We found that tight junctions are important for the establishment of a functional barrier-forming layer but impairing them does not reduce the mechanical integrity of cells. Depletion of ZO-1 results in a weak increase in cortical tension. An opposite effect was observed for disruption of E-cadherin-mediated adherens junctions using DTT. Opening of adherens junctions leads to substantial alterations of cellular mechanics such as reduced overall stiffness, but these changes turned out to be reversible after re-establishing disulfide bridges in E-cadherin by removal of DTT. We found that regulatory mechanisms exist that preserve mechanical integrity during recovery of disrupted adherens junctions.
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影响因子：
19
作者：
Diz-Muñoz A;Fletcher DA;Weiner OD
通讯作者：
Weiner OD
影响因子：
19
作者：
Brasch J;Harrison OJ;Honig B;Shapiro L
通讯作者：
Shapiro L
DOI：
10.1083/jcb.200612080
发表时间：
2007-03-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ikenouchi J;Umeda K;Tsukita S;Furuse M;Tsukita S
通讯作者：
Tsukita S
影响因子：
1.6
作者：
HUTTER, JL;BECHHOEFER, J
通讯作者：
BECHHOEFER, J
DOI：
10.1126/science.1254211
发表时间：
2014-10-31
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Buckley CD;Tan J;Anderson KL;Hanein D;Volkmann N;Weis WI;Nelson WJ;Dunn AR
通讯作者：
Dunn AR