Thermally mediated double emulsion droplets formation in a six-way junction microfluidic device
Thermally mediated double emulsion droplets formation in a six-way junction microfluidic device
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六路连接微流体装置中热介导双乳液液滴的形成
DOI：
10.1016/j.colsurfa.2023.130961
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发表时间：
2023-03
期刊：
影响因子：
--
通讯作者：
Pei Zhou
中科院分区：
文献类型：
--
作者：
Jiajia Wu;Cai Gao;Dongfang Sun;Lei Yang;Bin Ye;Tao Wang;Pei Zhou
微米级液滴的可靠产生是基于液滴的微流体中各种应用的重要过程。平面流动聚焦技术是近年来出现的一种新型微滴产生技术。该技术可用于制备双乳液液滴，其具有单分散性高、尺寸可控、稳定性好等优点。然而，现有的基于该技术的制备工艺通常在环境温度下实施，并且主要通过调节流速来实现液滴的产生和尺寸调节。为了实现微滴制备过程中的精确温度控制，研制了一种基于定制热电制冷器的微流控芯片温度调节系统。考察了温度对复乳液滴生成稳定性、大小和频率的影响。结果表明，以二棕榈酰磷脂酰胆碱为壳材料，稳定生成复乳液滴的温度区间为0-40 °C。随着温度的升高，液滴的产生频率增加，而内、外径则呈现相反的趋势。结果表明，内水相的包封率先升高后降低，说明单纯提高温度并不能提高包封率。当温度降低到-5 °C左右时，内部流体相不能被中间流体相包裹，无法形成双重乳液液滴。当温度超过40 °C时，双重乳液液滴的产生变得不稳定。本研究的结果有助于更好地理解液滴微流控系统在不同温度下的双重乳液液滴生成过程，有望在低温生物学、细胞生物力学和药物学等领域得到实际应用。
The reliable generation of micron-sized droplets is an important process for various applications in droplet-based microfluidics. Planar flow-focusing is a novel microdroplet generation technique emerging in recent years. This technology can be used to generate double emulsion droplets, which have the advantages of high monodispersity, controllable size, and good robustness. However, the existing preparation processes based on this technique are often implemented at ambient temperature, and the droplet generation and size adjustment are realized mainly through regulating the flow rate. In this study, a temperature regulation system of microfluidic chip based on a customized thermoelectric cooler was developed to realize the accurate temperature control of the droplets preparation. The influences of temperature on the generation stability, size, and frequency of double emulsion droplets were investigated. With dipalmitoyl-phosphatidylcholine as the shell material, results showed that the temperature interval for the stable generation of double emulsion droplets was 0–40 °C. With the increasing temperature, the generation frequency of the droplets increased, while the inner and outer diameters show the opposite trend. As a result, the encapsulation efficiency of inner aqueous phase firstly increased and then decreased, indicating that the encapsulation productivity could not be improved simply through temperature elevating. When the temperature is reduced to around − 5 °C, the inner fluid phase could not be wrapped by the middle fluid phase, failing the formation of double emulsion droplets. When the temperature exceeds 40 °C, the generation of double emulsion droplets became unstable. The findings of this study can help for better understanding of double emulsion droplets generation in droplet microfluidic systems operating with different temperatures, which is expected to be practically used in such fields as cryobiology, cell biomechanics, and pharmaceutics.
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新型六路连接微流控装置双乳液形成及壳厚影响因素研究
DOI：
10.1016/j.colsurfa.2020.124917
发表时间：
2020-05
期刊：
Colloids and Surfaces A: Physicochemical and Engineering Aspects
影响因子：
--
作者：
Shiyao Tan;Cai Gao;Huanyu Liu;Bin Ye;Dongfang Sun
通讯作者：
Dongfang Sun
DOI：
10.1117/12.822762
发表时间：
2008-12
期刊：
--
影响因子：
--
作者：
Chao Han;Jing Cheng
通讯作者：
Chao Han;Jing Cheng
影响因子：
4.6
作者：
Y. Tseng;F. Tseng;Yu-Feng Chen;C. Chieng
通讯作者：
Y. Tseng;F. Tseng;Yu-Feng Chen;C. Chieng
影响因子：
4
作者：
N. Nguyen;T. H. Ting;Y. Yap;T. Wong;J. Chai;W. Ong;Jun Zhou;S. Tan;L. Yobas
通讯作者：
N. Nguyen;T. H. Ting;Y. Yap;T. Wong;J. Chai;W. Ong;Jun Zhou;S. Tan;L. Yobas
影响因子：
3.2
作者：
Teh, Shia-Yen;Khnouf, Ruba;Lee, Abraham P.
通讯作者：
Lee, Abraham P.