Bisphosphonates inhibit surface-mediated osteogenesis.
Bisphosphonates inhibit surface-mediated osteogenesis.
复制标题
DOI：
10.1002/jbm.a.36944
复制
发表时间：
2020-08-01
期刊：
影响因子：
--
通讯作者：
Schwartz Z
中科院分区：
文献类型：
--
作者：
Lotz EM;Lohmann CH;Boyan BD;Schwartz Z
双膦酸盐靶向破骨细胞，减缓骨吸收，从而为支持骨整合提供依据。然而，二膦酸盐可能会对成骨细胞产生负面影响，损害种植体周围骨形成。本研究的目的是评估双膦酸盐对成骨细胞表面介导的成骨作用的影响。MG 63细胞在15 mm 2级钛盘上培养：光滑PT、疏水-微粗糙SLA或疏水-微粗糙mSLA（Institut Straumann AG，巴塞尔，瑞士）。组织培养聚苯乙烯（TCPS）用作对照。在汇合时，细胞用0 M、10− 8 M、10− 7 M和10− 6 M的阿仑膦酸盐、唑来膦酸盐或伊班膦酸盐处理24小时。Sprague道利大鼠也用1μg/kg/天伊班膦酸钠或磷酸盐缓冲盐水对照治疗5周。颅骨成骨细胞（rOBs）分离，表征，并在表面上培养。使用ELISA定量培养基中的成骨标志物。双膦酸盐处理降低了MG 63细胞中的骨钙素、骨保护素、骨桥蛋白、BMP 2、PGE 2、TGFβ1、IL 10和VEGF。该效应在粗糙表面上更稳健，并且较高浓度的二膦酸盐抑制了TCPS/PT水平的产生。伊班膦酸盐处理的ROBs产生较少的成骨标志物，类似于直接双膦酸盐处理。这些结果表明双膦酸盐暴露危害成骨细胞对微结构表面的促成骨反应。它们的作用在体内持续存在，并在体外对成骨细胞的反应产生负面影响。临床上，双膦酸盐可能会损害骨整合。
Bisphosphonates target osteoclasts, slowing bone resorption thus providing rationale to support osseointegration. However, bisphosphonates may negatively affect osteoblasts, impairing peri-implant bone formation. The goal of this study was to assess the effects bisphosphonates have on surface mediated osteogenesis of osteoblasts. MG63 cells were cultured on 15mm grade 2 titanium disks: smooth PT, hydrophobic-microrough SLA, or hydrophilic-microrough mSLA (Institut Straumann AG, Basel, Switzerland). Tissue culture polystyrene (TCPS) was used as a control. At confluence, cells were treated with 0M, 10−8M, 10−7M, and 10−6M of alendronate, zoledronate, or ibandronate for 24h. Sprague Dawley rats were also treated with 1μg/kg/day ibandronate or phosphate buffered saline control for 5wk. Calvarial osteoblasts (rOBs) were isolated, characterized, and cultured on surfaces. Osteogenic markers in the media were quantified using ELISAs. Bisphosphonate treatment reduced osteocalcin, osteoprotegerin, osteopontin, BMP2, PGE2, TGFβ1, IL10, and VEGF in MG63 cells. The effect was more robust on rough surfaces, and higher concentrations of bisphosphonates stunted production to TCPS/PT levels. Ibandronate conditioned rOBs produced less osteogenic markers similar to direct bisphosphonate treatment. These results suggest bisphosphonate exposure jeopardizes the pro-osteogenic response osteoblasts have to microstructured surfaces. Their effects persist in vivo and negatively condition osteoblast response in vitro. Clinically, bisphosphonates could compromise osseointegration.
登录
查看更多内容
影响因子：
8.8
作者：
Mackie PS;Fisher JL;Zhou H;Choong PF
通讯作者：
Choong PF
影响因子：
4
作者：
Indridason, OS;Franzson, L;Sigurdsson, G
通讯作者：
Sigurdsson, G
影响因子：
3.9
作者：
Corrado, Addolorata;Neve, Anna;Cantatore, Francesco Paolo
通讯作者：
Cantatore, Francesco Paolo
DOI：
10.1002/jbm.10290
发表时间：
2002-11-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Lohmann, CH;Tandy, EM;Schwartz, Z
通讯作者：
Schwartz, Z
影响因子：
4.9
作者：
Lotz, Ethan M.;Olivares-Navarrete, Rene;Schwartz, Zvi
通讯作者：
Schwartz, Zvi