Comparison of microfabricated hexagonal and lamellar post arrays for DNA electrophoresis.
Comparison of microfabricated hexagonal and lamellar post arrays for DNA electrophoresis.
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DOI：
10.1002/elps.201300381
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发表时间：
2014-03
期刊：
影响因子：
2.9
通讯作者：
Dorfman, Kevin D.
中科院分区：
文献类型：
--
作者：
Chen, Zhen;Dorfman, Kevin D.
我们使用布朗动力学模拟比较DNA分离的微制造后阵列包含无论是六边形或层状晶格。与直觉相反，密集的六边形阵列与频繁的DNA后碰撞不产生最佳分离。相反，具有与DNA的回转半径相称的孔径的六边形阵列由于随分子量增加的分子量依赖性碰撞概率而导致分离分辨率增加。然而，当六边形阵列太稀疏时，由于碰撞次数少，这种优势就丧失了。层状晶格，如DNA纳米围栏，似乎是上级在相同的后密度的六边形阵列，因为层状晶格结合了区域的DNA松弛与局部密集的后区域的碰撞。不同的后阵列设计的相对优势解释的碰撞次数和滞留时间的统计方面，提供指导原则，设计后阵列分离长DNA。
We used Brownian dynamics simulations to compare DNA separations in microfabricated post arrays containing either hexagonal or lamellar lattices. Contrary to intuition, dense hexagonal arrays with frequent DNA-post collisions do not yield the optimal separation. Rather, hexagonal arrays with pore sizes commensurate with the radius of gyration of the DNA lead to increased separation resolution due to a molecular-weight dependent collision probability that increases with molecular weight. However, when the hexagonal array is too sparse, this advantage is lost due to the low number of collisions. Lamellar lattices, such as the DNA nanofence, appear to be superior to a hexagonal array at the same post density, since the lamellar lattice combines regions for DNA relaxation with locally dense post regions for collisions. The relative advantages of different post array designs are explained in terms of the statistics for the number of collisions and the holdup time, providing guidelines for designing post arrays for separating long DNA.
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