Nutrient induced fluorescence transients (NIFTs) provide a rapid measure of P and C (co-)limitation in a green alga
Nutrient induced fluorescence transients (NIFTs) provide a rapid measure of P and C (co-)limitation in a green alga
复制标题
营养诱导荧光瞬变 (NIFT) 可快速测量绿藻中的 P 和 C(共)限制
DOI:
10.1080/09670262.2015.1095355
复制
发表时间:
2016
影响因子:
2.4
通讯作者:
J. Beardall
中科院分区:
文献类型:
--
作者:
Spijkerman;S. Stojkovic;D. Holland;S.C. Lachmann ;J. Beardall
Nutrient Induced Fluorescence Transients (NIFTs) have been shown to be a possible way of testing for the limiting nutrient in algal populations. In this study we tested the hypothesis that NIFTs can be used to detect a (co-)limitation for inorganic phosphorus (Pi) and CO2in the green algaChlamydomonas acidophilaand that the magnitude of the NIFTs can be related to cellular P:C ratios. We show a co-limitation response for Pi and CO2via traditional nutrient enrichment experiments in natural phytoplankton populations dominated byC. acidophila. We measured NIFT responses after a Pi- or a CO2-spike inC. acidophilabatch cultures at various stages of Pi and inorganic C limitation. Significant NIFTs were observed in response to spikes in both nutrients. The NIFT response to a Pi-spike showed a strong negative correlation with cellular P:C ratio that was pronounced below 3 mmol P: mol C (equivalent to 0.2 pg P cell–1). Both cellular P and C content influenced the extent of the Pi-NIFT response. The NIFT response to a CO2-spike correlated to low CO2culturing conditions and also had a negative correlation with cellular P content. A secondary response within the Pi-NIFT response was related to the CO2concentration and potentially reflected co-limitation. In conclusion, NIFTs provided a quick and reliable method to detect the growth-limiting nutrient in an extremophile green alga, under Pi-, CO2-and Pi/CO2(co-)limited growth conditions.
登录
查看更多内容
影响因子:
3.7
作者:
Spijkerman E;de Castro F;Gaedke U
通讯作者:
Gaedke U
DOI:
10.1071/pp9950331
发表时间:
1995
期刊:
Australian Journal of Plant Physiology
影响因子:
--
作者:
Wood;R. Oliver
通讯作者:
R. Oliver
影响因子:
2.9
作者:
S. Roberts;Kirsten Shelly;J. Beardall
通讯作者:
J. Beardall
影响因子:
6.4
作者:
S. Mellvig;J. Tillberg
通讯作者:
J. Tillberg
DOI:
--
发表时间:
--
期刊:
影响因子:
--
作者:
通讯作者:
--