Nutrient induced fluorescence transients (NIFTs) provide a rapid measure of P and C (co-)limitation in a green alga

Nutrient induced fluorescence transients (NIFTs) provide a rapid measure of P and C (co-)limitation in a green alga
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营养诱导荧光瞬变 (NIFT) 可快速测量绿藻中的 P 和 C(共)限制

DOI:
10.1080/09670262.2015.1095355
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发表时间:
2016
影响因子:
2.4
通讯作者:
J. Beardall
J. Beardall
中科院分区:
生物学3区
文献类型:
--
作者:
Spijkerman;S. Stojkovic;D. Holland;S.C. Lachmann ;J. Beardall

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营养诱导荧光瞬态(NIFT)已被证明是一种可能的方法来测试的限制营养藻类种群。在这项研究中,我们测试的假设,NIFTs可以用来检测(共同)限制无机磷(Pi)和二氧化碳的绿色藻类嗜酸衣原体和NIFTs的幅度可以与细胞的P:C比。我们通过传统的营养盐富集实验,在以C.嗜酸菌我们测量了在C中Pi或CO2峰值后的NIFT响应。在不同阶段的Pi和无机C限制的嗜酸性培养物。观察到显着的NIFTs响应这两种营养素的峰值。NIFT对Pi尖峰的响应显示出与细胞P:C比率的强负相关性,其在低于3 mmol P:mol C(相当于0.2 pg P细胞-1)时是明显的。细胞P和C含量影响Pi-NIFT反应的程度。NIFT对CO2峰值的响应与低CO2培养条件相关,也与细胞P含量呈负相关。Pi-NIFT响应中的次要响应与CO2浓度相关,可能反映了共同限制。总之,NIFTs提供了一种快速可靠的方法来检测生长限制营养素在极端嗜绿色菌,在Pi-,CO2-和Pi/CO2(共)限制生长条件。
Nutrient Induced Fluorescence Transients (NIFTs) have been shown to be a possible way of testing for the limiting nutrient in algal populations. In this study we tested the hypothesis that NIFTs can be used to detect a (co-)limitation for inorganic phosphorus (Pi) and CO2in the green algaChlamydomonas acidophilaand that the magnitude of the NIFTs can be related to cellular P:C ratios. We show a co-limitation response for Pi and CO2via traditional nutrient enrichment experiments in natural phytoplankton populations dominated byC. acidophila. We measured NIFT responses after a Pi- or a CO2-spike inC. acidophilabatch cultures at various stages of Pi and inorganic C limitation. Significant NIFTs were observed in response to spikes in both nutrients. The NIFT response to a Pi-spike showed a strong negative correlation with cellular P:C ratio that was pronounced below 3 mmol P: mol C (equivalent to 0.2 pg P cell–1). Both cellular P and C content influenced the extent of the Pi-NIFT response. The NIFT response to a CO2-spike correlated to low CO2culturing conditions and also had a negative correlation with cellular P content. A secondary response within the Pi-NIFT response was related to the CO2concentration and potentially reflected co-limitation. In conclusion, NIFTs provided a quick and reliable method to detect the growth-limiting nutrient in an extremophile green alga, under Pi-, CO2-and Pi/CO2(co-)limited growth conditions.
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