Ultraflexible and Stretchable Intrafascicular Peripheral Nerve Recording Device with Axon-Dimension, Cuff-Less Microneedle Electrode Array.
Ultraflexible and Stretchable Intrafascicular Peripheral Nerve Recording Device with Axon-Dimension, Cuff-Less Microneedle Electrode Array.
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DOI：
10.1002/smll.202200311
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发表时间：
2022-05
期刊：
影响因子：
13.3
通讯作者：
Seymour, John P.
中科院分区：
文献类型：
--
作者：
Yan, Dongxiao;Jiman, Ahmad A.;Bottorff, Elizabeth C.;Patel, Paras R.;Meli, Dilara;Welle, Elissa J.;Ratze, David C.;Havton, Leif A.;Chestek, Cynthia A.;Kemp, Stephen W. P.;Bruns, Tim M.;Yoon, Euisik;Seymour, John P.
关键词：
需要具有更高空间分辨率的周围神经标测工具来推动系统神经科学的发展，并有可能在神经调节应用中提供一个闭合环生物标记物。微尺度神经接口的两个关键挑战是(I)如何将其应用于小的周围神经，以及(Ii)如何将慢性反应性降至最低。我们研制了柔性微针神经阵列(MINA)，这是第一个用轴突大小的硅微针嵌入低模数硅胶制成的高密度穿透电极阵列。我们介绍了植入的MINA的设计、制造、急性记录和慢性反应。在大鼠腓神经中发现了不同的单位。我们还展示了一种长期的、无袖套和无缝线的固定方式，使用孟加拉玫瑰作为光激活粘合剂，持续两个时间点。观察1周和6周时的组织反应，包括两个假手术组(N=5和N=3)和两个Mina植入组(N=5和N=4)。用组织形态计量学的方法对大鼠左侧迷走神经中的这些情况进行量化。添加了Micro-CT以可视化和量化种植体周围的组织包裹物。MINA显示，与以前量化的束间方法相比，包膜厚度减少了。未来的挑战包括精确插入微针电极的技术和展示长期记录的技术。研制了一种超小型微针神经阵列，用于周围神经的标测研究。各向同性和各向异性反应腐蚀形成每个电极。该工艺允许包含高温二氧化硅绝缘材料和符合要求的医用级弹性体基材。无袖带和顺应性的神经界面通过光化学激活与体内的自主神经相连。结果表明，制造，急性电生理，和高保真的设备-组织接口。
Peripheral nerve mapping tools with higher spatial resolution are needed to advance systems neuroscience, and potentially provide a closed-loop biomarker in neuromodulation applications. Two critical challenges of microscale neural interfaces are (i) how to apply them to small peripheral nerves, and (ii) how to minimize chronic reactivity. We developed a flexible microneedle nerve array (MINA), which is the first high-density penetrating electrode array made with axon-sized silicon microneedles embedded in low-modulus thin silicone. We present the design, fabrication, acute recording, and chronic reactivity to an implanted MINA. Distinctive units were identified in the rat peroneal nerve. We also demonstrate a long-term, cuff-free, and suture-free fixation manner using rose bengal as a light-activated adhesive for two timepoints. The tissue response was investigated at 1-week and 6-week timepoints, including two sham groups (N=5 and N=3, respectively) and two MINA-implanted groups (N=5 and N=4, respectively). These conditions were quantified in the left vagus nerve of rats using histomorphometry. Micro-CT was added to visualize and quantify tissue encapsulation around the implant. MINA demonstrated a reduction in encapsulation thickness over previously quantified interfascicular methods. Future challenges include techniques for precise insertion of the microneedle electrodes and demonstrating long-term recording. An ultrasmall microneedle nerve array is developed for mapping studies in peripheral nerves. Isotropic and anisotropic reactive etching shape each electrode. This process allows the inclusion of high-temperature silicon dioxide insulation and a compliant medical-grade elastomer substrate. The cuffless and compliant nerve interface is attached to autonomic nerves in vivo using photochemical activation. The results demonstrate fabrication, acute electrophysiology, and a high-fidelity device-tissue interface.
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影响因子：
38.3
作者：
Liu J;Fu TM;Cheng Z;Hong G;Zhou T;Jin L;Duvvuri M;Jiang Z;Kruskal P;Xie C;Suo Z;Fang Y;Lieber CM
通讯作者：
Lieber CM
影响因子：
2.2
作者：
Borschel, GH;Kia, KF;Dennis, RG
通讯作者：
Dennis, RG
影响因子：
14
作者：
Christensen, Michael B.;Wark, Heather A. C.;Hutchinson, Douglas T.
通讯作者：
Hutchinson, Douglas T.
影响因子：
4.6
作者：
McCallum GA;Sui X;Qiu C;Marmerstein J;Zheng Y;Eggers TE;Hu C;Dai L;Durand DM
通讯作者：
Durand DM
影响因子：
4.6
作者：
HETKE, JF;LUND, JL;ANDERSON, DJ
通讯作者：
ANDERSON, DJ