Essential Oils Produce Developmental Toxicity in Zebrafish Embryos and Cause Behavior Changes in Zebrafish Larvae.
Essential Oils Produce Developmental Toxicity in Zebrafish Embryos and Cause Behavior Changes in Zebrafish Larvae.
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精油在斑马鱼胚胎中产生发育毒性，并导致斑马鱼幼虫的行为变化。
DOI：
10.3390/biomedicines11102821
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发表时间：
2023-10-18
期刊：
影响因子：
4.7
通讯作者：
中科院分区：
文献类型：
--
作者：
由于其生物特性，精油在各个行业中都很受欢迎，但对其对生物体的潜在毒性影响的研究却很少。本研究旨在评价柠檬草、百里香和牛至精油对斑马鱼胚胎和幼虫的动物模型的影响。胚胎暴露于不同浓度的精油中，并评估各种终点，包括表观代谢、死亡率（LC50）、形态计量学和行为变化。这三种精油都能减少表观代谢，影响胚胎发育。计算柠檬草（3.7µg/mL）、百里香（14.4µg/mL）和牛至（5.3µg/mL）油的LC50值。接触这些油的幼虫表现出形态缺陷，包括生长减少、脊柱变形、心包水肿、眼睛大小缩小和膀胱肿胀减少。形态计量学分析证实，较高的油浓度降低了幼虫的长度。精油暴露改变了斑马鱼幼虫的游泳行为，柠檬草油减少了暗周期活动，牛至油增加了光周期活动，表明神经发育毒性。这些发现说明了这些精油对斑马鱼胚胎和幼虫的不利影响，并揭示了精油的毒性，表明应谨慎使用，特别是在怀孕期间。
Essential oils have gained significant popularity in various industries due to their biological properties, but their potential toxic effects on living organisms have been poorly investigated. This study aimed to evaluate the effects of lemongrass, thyme, and oregano essential oils on zebrafish embryos and larvae as animal models. Embryos were exposed to different concentrations of essential oils, and various endpoints were assessed, including epiboly, mortality (LC50), morphometry, and behavioral changes. All three essential oils reduced epiboly, affecting embryonic development. LC50 values were calculated for lemongrass (3.7 µg/mL), thyme (14.4 µg/mL), and oregano (5.3 µg/mL) oils. Larvae exposed to these oils displayed morphological defects, including growth reduction, spinal deformation, pericardial edema, eye size reduction, and reduced swim-bladder inflation. Morphometric analysis confirmed reduced larval length at higher oil concentrations. Essential-oil exposure altered zebrafish larval swimming behavior, with lemongrass oil reducing dark-cycle activity and oregano oil increasing light-cycle activity, suggesting neurodevelopmental toxicity. These findings illustrate the adverse effects of these oils on zebrafish embryos and larvae and reveal essential-oil toxicity, indicating careful use should be considered, particularly during pregnancy.
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影响因子：
2.9
作者：
Sales Cadena MR;Cadena PG;Watson MR;Sarmah S;Boehm Ii SL;Marrs JA
通讯作者：
Marrs JA
影响因子：
3
作者：
Smith, GD;Wilburn, C;McCarthy, RA
通讯作者：
McCarthy, RA
影响因子：
4.8
作者：
Fernandes, Yohaan;Rampersad, Mindy;Gerlai, Robert
通讯作者：
Gerlai, Robert
DOI：
10.3390/foods11030464
发表时间：
2022-02-04
期刊：
Foods (Basel, Switzerland)
影响因子：
--
作者：
Angane M;Swift S;Huang K;Butts CA;Quek SY
通讯作者：
Quek SY
影响因子：
4.6
作者：
Osaili, Tareq M.;Dhanasekaran, Dinesh Kumar;Zeb, Falak;Faris, MoezAlIslam E.;Naja, Farah;Radwan, Hadia;Ismail, Leila Cheikh;Hasan, Hayder;Hashim, Mona;Obaid, Reyad Shaker
通讯作者：
Obaid, Reyad Shaker