Enhanced cell ingrowth and proliferation through three-dimensional nanocomposite scaffolds with controlled pore structures.
Enhanced cell ingrowth and proliferation through three-dimensional nanocomposite scaffolds with controlled pore structures.
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DOI：
10.1021/bm901260y
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发表时间：
2010-03-08
影响因子：
6.2
通讯作者：
Lu, Lichun
中科院分区：
文献类型：
--
作者：
Lee, Kee-Won;Wang, Shanfeng;Dadsetan, Mahrokh;Yaszemski, Michael J.;Lu, Lichun
我们通过使用聚富马酸丙烯（PPF）和羟基磷灰石（HA）纳米颗粒制成的交联三维（3D）纳米复合材料支架来增强细胞的生长和增殖。采用计算机辅助设计（CAD）模型和固体自由成形（SFF）技术制备具有可控内部孔隙结构的支架，采用NaCl浸出技术制备具有随机孔隙结构的支架进行比较。采用扫描电镜（SEM）和力学性能测试对支架的形貌和力学性能进行了表征。采用x射线微计算机断层扫描（micro-CT）和三维成像分析评估支架的孔隙连通性。体外细胞研究使用MC3T3-E1小鼠成骨前细胞和培养支架在旋转壁血管生物反应器中进行4天和7天的体外细胞研究，以评估细胞的附着、活力、长入深度和增殖。添加透明质酸或改变孔隙结构后，交联纳米复合材料的力学性能无显著差异。然而，控制孔隙结构的PPF/HA纳米复合支架的孔隙连通性显著增加，导致细胞播种后7天细胞长入深度增强。在PPF/HA纳米复合支架中，细胞的附着和增殖也更高。这些结果表明，具有可控孔隙结构的交联PPF/HA纳米复合支架可能成为具有良好细胞增殖和长入性的骨组织工程支架。
We present enhanced cell ingrowth and proliferation through crosslinked three-dimensional (3D) nanocomposite scaffolds fabricated using poly(propylene fumarate) (PPF) and hydroxyapatite (HA) nanoparticles. Scaffolds with controlled internal pore structures were produced from computer-aided design (CAD) models and solid freeform fabrication (SFF) technique, while those with random pore structures were fabricated by NaCl leaching technique for comparison. The morphology and mechanical properties of scaffolds were characterized using scanning electron microscopy (SEM) and mechanical testing, respectively. Pore interconnectivity of scaffolds was assessed using X-ray micro-computed tomography (micro-CT) and 3D imaging analysis. In vitro cell studies have been performed using MC3T3-E1 mouse preosteoblasts and cultured scaffolds in a rotating-wall-vessel bioreactor for 4 and 7 days to assess cell attachment, viability, ingrowth depth, and proliferation. The mechanical properties of crosslinked nanocomposite scaffolds were not significantly different after adding HA or varying pore structures. However, pore interconnectivity of PPF/HA nanocomposite scaffolds with controlled pore structures has been significantly increased, resulting in enhanced cell ingrowth depth 7 days after cell seeding. Cell attachment and proliferation are also higher in PPF/HA nanocomposite scaffolds. These results suggest that crosslinked PPF/HA nanocomposite scaffolds with controlled pore structures may lead to promising bone tissue engineering scaffolds with excellent cell proliferation and ingrowth.
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影响因子：
14
作者：
van Lenthe, G. Harry;Hagenmueller, Henri;Mueller, Ralph
通讯作者：
Mueller, Ralph
DOI：
10.1111/j.1749-6632.2002.tb03061.x
发表时间：
2002-01-01
期刊：
REPARATIVE MEDICINE: GROWING TISSUES AND ORGANS
影响因子：
--
作者：
Chu, TMG;Hollister, SJ;Orton, DG
通讯作者：
Orton, DG
DOI：
10.1002/jbm.10150
发表时间：
2002-10-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Sikavitsas, VI;Bancroft, GN;Mikos, AG
通讯作者：
Mikos, AG
影响因子：
14
作者：
Ishaug-Riley, SL;Crane-Kruger, GM;Mikos, AG
通讯作者：
Mikos, AG
影响因子：
4.9
作者：
Simon, Joshua L.;Rekow, E. Dianne;Parsons, J. Russell
通讯作者：
Parsons, J. Russell