Paradoxical expressions of natriuretic peptide receptor-C and neutral endopeptidase account for C-type natriuretic peptide decline during the progression of experimental obstructive nephropathy
Paradoxical expressions of natriuretic peptide receptor-C and neutral endopeptidase account for C-type natriuretic peptide decline during the progression of experimental obstructive nephropathy
复制标题
实验性梗阻性肾病进展过程中利钠肽受体-C 和中性肽链内切酶的矛盾表达导致 C 型利钠肽下降
DOI：
10.1177/1470320313507121
复制
发表时间：
2014-12
影响因子：
2.9
通讯作者：
胡鹏
中科院分区：
文献类型：
--
作者：
胡鹏
简介：C型利钠肽（CNP）选择性结合鸟苷酸环化酶偶联利钠肽受体（NPR）-B，并发挥更有效的抗肥大和抗纤维化特性。CNP的消除主要通过中性内肽酶（NEP）和NPR-C进行。研究方法：建立单侧输尿管梗阻（UUO）大鼠模型，观察CNP在梗阻肾组织中表达的动态变化，并探讨NPR-B、NPR-C、NEP与CNP表达的相关性。结果如下：实时定量PCR和Western blot分析显示，与假手术组相比，结扎后即刻，梗阻肾组织中CNP mRNA和蛋白的表达量有升高趋势，而在随后的时间点下降。相关分析显示CNP mRNA与NPR-B mRNA呈正相关（r=+0.673，p = 0.05）。此外，NPR-C（mRNA和蛋白质分别为r=−0.943和−0.837，p<0.05）和NEP（mRNA和蛋白质分别为r=−0.687和−0.823，p<0.05）的表达增加伴随CNP显著下降。结论：高水平的CNP可能参与了UUO早期NPR-B表达的增加。更有趣的是，NPR-C和NEP的矛盾表达可能解释了阻塞肾中CNP的下降。
Introduction: C-type natriuretic peptide (CNP) selectively binds to the guanylyl cyclase coupled natriuretic peptide receptor (NPR)-B and exerts more potent antihypertrophic and antifibrotic properties. Elimination of CNP occurs mainly by neutral endopeptidase (NEP) and NPR-C. Methods: We established a rat model of unilateral ureteral obstruction (UUO) to examine the continuous change of the CNP expression and to assess the correlations of NPR-B, NPR-C, NEP with CNP in the obstructed kidneys. Results: The expressions of CNP mRNA and protein in the obstructed kidneys tended to be higher immediately after ligation and declined at later time points compared to sham-operated rats, measured by real-time polymerase chain reaction (PCR) and western blot analysis. Subsequent correlation analysis indicated that CNP mRNA was positively correlated with NPR-B mRNA (r=+0.673, p<0.05). In addition, the increased expression of NPR-C (r=−0.943 and −0.837 for mRNA and protein respectively, p<0.05) and NEP (r=−0.687 and −0.823 for mRNA and protein respectively, p<0.05) were accompanied by a significant decline in CNP. Conclusions: A high level of CNP may contribute to the elevated expression of NPR-B in the early phase of UUO. More interestingly, paradoxical expressions of NPR-C and NEP may account for the decline of CNP in the obstructed kidneys.
登录
查看更多内容
影响因子：
2.9
作者：
Robinder S. Garcha;A. Hughes
通讯作者：
Robinder S. Garcha;A. Hughes
DOI：
10.1677/joe.0.1580035
发表时间：
1998-07
期刊：
The Journal of endocrinology
影响因子：
--
作者：
S. Shin;J. Wen;Y. J. Lee;I. Chen;J. Tsai
通讯作者：
S. Shin;J. Wen;Y. J. Lee;I. Chen;J. Tsai
DOI：
10.1016/s0735-1097(02)02127-7
发表时间：
2002-09-18
影响因子：
24
作者：
Chen, HH;Lainchbury, JG;Burnett, JC
通讯作者：
Burnett, JC
DOI：
--
发表时间：
2006
期刊：
The Korean Journal of Physiology and Pharmacology
影响因子：
--
作者：
E. Bae;S. Kim
通讯作者：
E. Bae;S. Kim
尿C型利钠肽排泄增加可能是肾小管间质纤维化的早期标志
影响因子：
3
作者：
Hu, Peng;Wang, Jing;Qin, Yuan Han
通讯作者：
Qin, Yuan Han