17beta-estradiol promotes the odonto/osteogenic differentiation of stem cells from apical papilla via mitogen-activated protein kinase pathway.
17beta-estradiol promotes the odonto/osteogenic differentiation of stem cells from apical papilla via mitogen-activated protein kinase pathway.
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17β-雌二醇通过丝裂原激活蛋白激酶途径促进根尖乳头干细胞的牙向/成骨分化
DOI：
10.1186/scrt515
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发表时间：
2014-11-17
影响因子：
7.5
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Li Y;Yan M;Wang Z;Zheng Y;Li J;Ma S;Liu G;Yu J
雌激素在间充质干细胞的成骨分化中起重要作用，而根尖乳头干细胞（stem cells from apical papilla，SCAP）可促进牙本质/骨样组织的形成。迄今为止，雌激素对SCAP分化的影响尚不清楚。分离SCAP并用10-7 M 17 β-雌二醇（E2）处理。随后通过甲基噻唑基四氮唑（MTT）法等方法研究了成牙/成骨能力以及丝裂原活化蛋白激酶（MAPK）信号通路的参与。MTT法和流式细胞仪检测结果表明，E2对SCAP的体外增殖无影响，而碱性磷酸酶（ALP）活性测定和茜素红染色结果表明，E2能显著促进SCAP的ALP活性和矿化能力。实时荧光定量RT-PCR和western blot检测结果显示，E2处理后的SCAP中ALP、DMP 1/DMP 1、DSPP/DSP、RUNX 2/RUNX 2、OSX/OSX和OCN/OCN等成骨标志物表达均显著上调。此外，E2处理后，这些干细胞中磷酸化p38和磷酸化JNK的表达增强，核下游转录因子磷酸化Sp1、磷酸化Elk-1、磷酸化c-Jun和磷酸化c-Fos的表达也增强，表明MAPK信号通路在E2处理的SCAP向牙/成骨分化过程中被激活。相反，在MAPK特异性抑制剂的存在下，E2处理的SCAP的分化被抑制。10 ~（-7）M的17 β-雌二醇通过激活MAPK信号通路促进SCAP的内吞/成骨分化。
Estrogen plays an important role in the osteogenic differentiation of mesenchymal stem cells, while stem cells from apical papilla (SCAP) can contribute to the formation of dentin/bone-like tissues. To date, the effects of estrogen on the differentiation of SCAP remain unclear. SCAP was isolated and treated with 10-7 M 17beta-estradiol (E2). The odonto/osteogenic potency and the involvement of mitogen-activated protein kinase (MAPK) signaling pathway were subsequently investigated by using methyl-thiazolyl-tetrazolium (MTT) assay, and other methods. MTT and flow cytometry results demonstrated that E2 treatment had no effect on the proliferation of SCAP in vitro, while alkaline phosphatase (ALP) assay and alizarin red staining showed that E2 can significantly promote ALP activity and mineralization ability in SCAP. Real-time reverse transcription polymerase chain reaction (RT-PCR) and western blot assay revealed that the odonto/osteogenic markers (ALP, DMP1/DMP1, DSPP/DSP, RUNX2/RUNX2, OSX/OSX and OCN/OCN) were significantly upregulated in E2-treated SCAP. In addition, the expression of phosphor-p38 and phosphor-JNK in these stem cells was enhanced by E2 treatment, as was the expression of the nuclear downstream transcription factors including phosphor-Sp1, phosphor-Elk-1, phosphor-c-Jun and phosphor-c-Fos, indicating the activation of MAPK signaling pathway during the odonto/osteogenic differentiation of E2-treated SCAP. Conversely, the differentiation of E2-treated SCAP was inhibited in the presence of MAPK specific inhibitors. The ondonto/osteogenic differentiation of SCAP is enhanced by 10-7 M 17beta-estradiol via the activation of MAPK signaling pathway.
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影响因子：
4.8
作者：
Bodine, PVN;Henderson, RA;Komm, BS
通讯作者：
Komm, BS
雌激素受体β对人牙周膜细胞成骨分化功能的影响
影响因子：
3
作者：
Liang, Li;Yu, Ji-feng;Ding, Yin
通讯作者：
Ding, Yin
DOI：
10.1007/978-1-4419-1050-9_5
发表时间：
2010-01-01
期刊：
OSTEOIMMUNOLOGY: INTERACTIONS OF THE IMMUNE AND SKELETAL SYSTEMS II
影响因子：
--
作者：
Komori, Toshihisa
通讯作者：
Komori, Toshihisa
影响因子：
8
作者：
Ni P;Fu S;Fan M;Guo G;Shi S;Peng J;Luo F;Qian Z
通讯作者：
Qian Z
影响因子：
15.9
作者：
Kousteni, S;Han, L;Manolagas, SC
通讯作者：
Manolagas, SC