Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field.
Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field.
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DOI：
10.1111/pbi.12731
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发表时间：
2017-11
影响因子：
13.8
通讯作者：
Ishitani M
中科院分区：
文献类型：
--
作者：
Selvaraj MG;Ishizaki T;Valencia M;Ogawa S;Dedicova B;Ogata T;Yoshiwara K;Maruyama K;Kusano M;Saito K;Takahashi F;Shinozaki K;Nakashima K;Ishitani M
干旱胁迫往往导致作物产量大幅下降，这可能与全球变暖有关。在不影响粮食产量的情况下提高抗旱性一直是作物改良中的一大挑战。在此，我们报道了拟南芥半乳糖醇合成酶2基因(AtGolS2)在旱地条件下对两个不同水稻品种的耐旱性和产量的影响。在构建的玉米泛素启动子(Ubi：AtGolS2)控制下表达AtGolS2的转基因株系也比非转基因对照具有更高的半乳糖醇水平。干旱条件下转基因水稻产量的提高与穗数、籽粒肥力和生物量的增加有关。利用Ubi：AtGolS2转基因水稻品系在柯林加、热带水稻和NERICA4种间杂交种的田间田间试验表明，经过验证的品系具有Ubi：AtGolS2转基因水稻的田间耐旱性。改良后的抗旱性与叶片相对含水量高、光合作用活性高、植株生长减慢较少、恢复能力较快有关。总而言之，我们的结果提供了强有力的证据，表明在田间干旱胁迫下，AtGolS2是一种有效的生物技术工具，可以减少水稻在遗传差异之外的产量损失。
Drought stress has often caused significant decreases in crop production which could be associated with global warming. Enhancing drought tolerance without a grain yield penalty has been a great challenge in crop improvement. Here, we report the Arabidopsis thaliana galactinol synthase 2 gene (AtGolS2) was able to confer drought tolerance and increase grain yield in two different rice (Oryza sativa) genotypes under dry field conditions. The developed transgenic lines expressing AtGolS2 under the control of the constitutive maize ubiquitin promoter (Ubi:AtGolS2) also had higher levels of galactinol than the non‐transgenic control. The increased grain yield of the transgenic rice under drought conditions was related to a higher number of panicles, grain fertility and biomass. Extensive confined field trials using Ubi:AtGolS2 transgenic lines in Curinga, tropical japonica and NERICA4, interspecific hybrid across two different seasons and environments revealed the verified lines have the proven field drought tolerance of the Ubi:AtGolS2 transgenic rice. The amended drought tolerance was associated with higher relative water content of leaves, higher photosynthesis activity, lesser reduction in plant growth and faster recovering ability. Collectively, our results provide strong evidence that AtGolS2 is a useful biotechnological tool to reduce grain yield losses in rice beyond genetic differences under field drought stress.
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影响因子：
5.4
作者：
Gur, Amit;Osorio, Sonia;Fridman, Eyal;Fernie, Alisdair R.;Zamir, Dani
通讯作者：
Zamir, Dani
影响因子：
7.4
作者：
Oh, Se-Jun;Kim, Youn Shic;Kim, Ju-Kon
通讯作者：
Kim, Ju-Kon
DOI：
10.1051/agro:2008021
发表时间：
2009-01-01
期刊：
SUSTAINABLE AGRICULTURE
影响因子：
--
作者：
Farooq, M.;Wahid, A.;Basra, S. M. A.
通讯作者：
Basra, S. M. A.
影响因子：
6.9
作者：
Manavalan LP;Chen X;Clarke J;Salmeron J;Nguyen HT
通讯作者：
Nguyen HT
影响因子：
6.4
作者：
Collett, H;Shen, A;Illing, N
通讯作者：
Illing, N