hi2-1, a QTL which improves harvest index, earliness and alters metabolite accumulation of processing tomatoes.
hi2-1, a QTL which improves harvest index, earliness and alters metabolite accumulation of processing tomatoes.
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DOI：
10.1007/s00122-010-1412-8
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发表时间：
2010-11
影响因子：
5.4
通讯作者：
Zamir, Dani
中科院分区：
文献类型：
--
作者：
Gur, Amit;Osorio, Sonia;Fridman, Eyal;Fernie, Alisdair R.;Zamir, Dani
收获指数是指番茄的生殖产量与番茄总生物量的比值，早熟性是加工番茄的重要农艺性状。白英渐渗系群体表现出收获指数和早熟性的变异。这些性状的QTL大多数显示负的农艺效应，然而，hi 2 -1是一个独特的QTL，显示改善收获指数和早熟性。这种基因渗入在几年内和不同的遗传背景下进行了测试。在18 cM TG 33-TG 276区间分离的31个近等基因亚系用于该多表型QTL的精细定位。在此基础上，将株重、锤度、总产量和早熟性的表型效应共定位到同一区域。在不同的作图实验中，将这些亚系作为杂合子进行测试，以定位仅在杂合状态下表达的收获指数QTL。这些QTL定位到同一候选区域，表明hi 2 -1是一个单一的基因与多效性效应或代表连锁基因独立影响这些性状。果皮的代谢产物分析表明，一些代谢QTL与收获指数性状共分离，包括那些重要的运输同化物，如糖和氨基酸。对这些品系的开花模式的分析揭示了IL 2 -1植物的诱导开花，表明hi 2 -1也可能通过改变植物结构和开花速率来影响收获指数和早熟。本文的在线版本（doi：10.1007/s 00122 -010-1412-8）包含补充材料，可供授权用户使用。
Harvest index, defined as the ratio of reproductive yield to total plant biomass, and early ripening are traits with important agronomic value in processing tomatoes. The Solanum pennellii introgression-line (IL) population shows variation for harvest index and earliness. Most of the QTL mapped for these traits display negative agronomic effects; however, hi2-1 is a unique QTL displaying improved harvest index and earliness. This introgression was tested over several years and under different genetic backgrounds. Thirty-one nearly isogenic sub-lines segregating for the 18 cM TG33–TG276 interval were used for fine mapping of this multi-phenotypic QTL. Based on this analysis the phenotypic effects for plant weight, Brix, total yield and earliness were co-mapped to the same region. In a different mapping experiment these sub-lines were tested as heterozygotes in order to map the harvest index QTL which were only expressed in the heterozygous state. These QTL mapped to the same candidate region, suggesting that hi2-1 is either a single gene with pleiotropic effects or represents linked genes independently affecting these traits. Metabolite profiling of the fruit pericarp revealed that a number of metabolic QTL co-segregate with the harvest index trait including those for important transport assimilates such as sugars and amino acids. Analysis of the flowering pattern of these lines revealed induced flowering at IL2-1 plants, suggest that hi2-1 may also affect harvest index and early ripening by changing plant architecture and flowering rate. The online version of this article (doi:10.1007/s00122-010-1412-8) contains supplementary material, which is available to authorized users.
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