Analysis of ovary-specific genes in relation to egg maturation and female nutritional condition in the mosquitoes Georgecraigius atropalpus and Aedes aegypti (Diptera: Culicidae).
Analysis of ovary-specific genes in relation to egg maturation and female nutritional condition in the mosquitoes Georgecraigius atropalpus and Aedes aegypti (Diptera: Culicidae).
复制标题
DOI：
10.1016/j.jinsphys.2012.11.006
复制
发表时间：
2013-03
影响因子：
2.2
通讯作者：
Donnell, David M.
中科院分区：
文献类型：
--
作者：
Telang, Aparna;Rechel, Julie A.;Brandt, Jessica R.;Donnell, David M.
关键词：
在分子水平上分析自体蚊子的生殖生理学是复杂的，因为卵巢成熟的不稳定性和对血餐的消化。与自体蚊子相比，自体雌蚊在幼虫期可以获得更多的营养物质，并且在成虫羽化时卵巢分泌的蜕皮激素也更高。这些特征基本上取代了血粉在提供第一批卵子和启动卵子发育方面的作用。为了深入了解卵巢成熟的过程，我们首先进行了一个转录本分析的强制性自体蚊子Georgecraigius atropalpus（以前Ochlerotatus atropalpus）。我们确定EST使用抑制性消减杂交（SSH）的成绩单从卵巢在卵子发生过程中的关键时刻在血液消化的情况下。对载脂蛋白III（APO）和氧固醇结合蛋白（OSBP）等基因表达的初步研究表明，这些基因可能与女性营养状况有关。然后，我们将我们的发现应用于医学上重要的自体蚊子埃及伊蚊。这些基因在Ae. Aegypti发现载脂蛋白O转录物的减少导致胴体和卵巢中脂质水平的降低，OSBP可能在整体脂质和固醇体内平衡中发挥作用。除了扩大我们对蚊子卵巢发育的理解外，继续使用自体和自体物种之间的比较方法可能会为蚊子产卵的调节提供新的干预点。
Analysis of the reproductive physiology of anautogenous mosquitoes at the molecular level is complicated by the simultaneity of ovarian maturation and the digestion of a blood meal. In contrast to anautogenous mosquitoes, autogenous female mosquitoes can acquire greater nutrient stores as larvae and exhibit higher ovarian production of ecdysteroids at adult eclosion. These features essentially replace the role of a blood meal in provisioning the first batch of eggs and initiating egg development. To gain insight into the process of ovary maturation we first performed a transcript analysis of the obligatory autogenous mosquito Georgecraigius atropalpus (formerly Ochlerotatus atropalpus). We identified ESTs using suppressive subtractive hybridization (SSH) of transcripts from ovaries at critical times during oogenesis in the absence of blood digestion. Preliminary expression studies of genes such as apolipophorin III (APO) and oxysterol binding protein (OSBP) suggested these genes might be cued to female nutritional status. We then applied our findings to the medically important anautogenous mosquito Aedes aegypti. RNAi-based analyses of these genes in Ae. aegypti revealed a reduction in APO transcripts leads to reduced lipid levels in carcass and ovaries and that OSBP may play a role in overall lipid and sterol homeostasis. In addition to expanding our understanding of mosquito ovarian development, the continued use of a comparative approach between autogenous and anautogenous species may provide novel intervention points for the regulation of mosquito egg production.
登录
查看更多内容
影响因子：
3.8
作者：
Murataliev, Marat B.;Guzov, Victor M.;Walker, F. Ann;Feyereisen, Rene
通讯作者：
Feyereisen, Rene
影响因子：
6.5
作者：
Kimmel AR;Brasaemle DL;McAndrews-Hill M;Sztalryd C;Londos C
通讯作者：
Londos C
影响因子：
4.4
作者：
Dana AN;Hong YS;Kern MK;Hillenmeyer ME;Harker BW;Lobo NF;Hogan JR;Romans P;Collins FH
通讯作者：
Collins FH
影响因子：
2.8
作者：
Gulia-Nuss, Monika;Eum, Jai-Hoon;Brown, Mark R.
通讯作者：
Brown, Mark R.
影响因子：
4.8
作者：
Brown, MR;Graf, R;Lea, AO
通讯作者：
Lea, AO