U-box E3 ubiquitin ligase PUB17 acts in the nucleus to promote specific immune pathways triggered by Phytophthora infestans.
U-box E3 ubiquitin ligase PUB17 acts in the nucleus to promote specific immune pathways triggered by Phytophthora infestans.
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U-box E3 泛素连接酶 PUB17 在细胞核中发挥作用，促进致病疫霉触发的特定免疫途径。
DOI：
10.1093/jxb/erv128
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发表时间：
2015-06
影响因子：
6.9
通讯作者：
Tian Z
中科院分区：
文献类型：
--
作者：
He Q;McLellan H;Boevink PC;Sadanandom A;Xie C;Birch PR;Tian Z
泛素E3连接酶PUB17在细胞核中发挥作用，在植物细胞表面感知特定激发子后，正向调节病原相关分子模式触发的免疫（PTI）中的转录反应以及程序性细胞死亡。
泛素化调节植物中的许多过程，包括免疫。E3泛素连接酶PUB17是番茄中由抗性蛋白CF4/9触发的程序性细胞死亡（PCD）的正调节因子。在此研究了它在对马铃薯晚疫病菌（Phytophthora infestans）免疫中的作用。通过RNA干扰使马铃薯中的StPUB17沉默以及通过病毒诱导的基因沉默（VIGS）使本氏烟（Nicotiana benthamiana）中的NbPUB17沉默，均增强了晚疫病菌在叶片上的定殖。由flg22激活的病原相关分子模式触发的免疫（PTI）转录反应以及由CF4/Avr4介导的PCD因PUB17沉默而减弱。然而，PUB17沉默并不影响由晚疫病菌病原相关分子模式INF1触发的PCD，或R3a/AVR3a的共表达，这表明并非所有与PTI和PCD相关的反应都需要PUB17。PUB17定位于植物细胞核，尤其在核仁中。显性负性的StPUB17V314I，V316I突变体（其保留核仁定位）的瞬时过表达抑制了CF4介导的细胞死亡并增强了晚疫病菌的定殖。将StPUB17V314I，V316I突变体排除在细胞核外消除了其显性负性活性，表明StPUB17在细胞核中发挥作用。PUB17是对晚疫病免疫的正调节因子，它在细胞核中起作用以促进特定的PTI和PCD途径。
Ubiquitin E3 ligase PUB17 functions in the nucleus to regulate transcriptional responses positively in PAMP-triggered immunity and programmed cell death following perception of specific elicitors at the plant cell surface. Ubiquitination regulates many processes in plants, including immunity. The E3 ubiquitin ligase PUB17 is a positive regulator of programmed cell death (PCD) triggered by resistance proteins CF4/9 in tomato. Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here. Silencing StPUB17 in potato by RNAi and NbPUB17 in Nicotiana benthamiana by virus-induced gene silencing (VIGS) each enhanced P. infestans leaf colonization. PAMP-triggered immunity (PTI) transcriptional responses activated by flg22, and CF4/Avr4-mediated PCD were attenuated by silencing PUB17. However, silencing PUB17 did not compromise PCD triggered by P. infestans PAMP INF1, or co-expression of R3a/AVR3a, demonstrating that not all PTI- and PCD-associated responses require PUB17. PUB17 localizes to the plant nucleus and especially in the nucleolus. Transient over-expression of a dominant-negative StPUB17V314I,V316I mutant, which retained nucleolar localization, suppressed CF4-mediated cell death and enhanced P. infestans colonization. Exclusion of the StPUB17V314I,V316I mutant from the nucleus abolished its dominant-negative activity, demonstrating that StPUB17 functions in the nucleus. PUB17 is a positive regulator of immunity to late blight that acts in the nucleus to promote specific PTI and PCD pathways.
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影响因子：
7.4
作者：
Liu, Jinling;Li, Wei;Wang, Guo-Liang
通讯作者：
Wang, Guo-Liang
DOI：
10.1126/science.1171647
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Boller T;He SY
通讯作者：
He SY
影响因子：
5.4
作者：
Block A;Alfano JR
通讯作者：
Alfano JR
影响因子：
6.7
作者：
Birch, Paul R. J.;Bryan, Glenn;Toth, Ian K.
通讯作者：
Toth, Ian K.
影响因子：
7.2
作者：
Gilroy, Eleanor M.;Hein, Ingo;Birch, Paul R. J.
通讯作者：
Birch, Paul R. J.