First insights into the genetic diversity of Mycobacterium tuberculosis isolates from HIV-infected Mexican patients and mutations causing multidrug resistance.
First insights into the genetic diversity of Mycobacterium tuberculosis isolates from HIV-infected Mexican patients and mutations causing multidrug resistance.
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DOI：
10.1186/1471-2180-10-82
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发表时间：
2010-03-17
期刊：
影响因子：
4.2
通讯作者：
Gonzalez-y-Merchand JA
中科院分区：
文献类型：
--
作者：
Lopez-Alvarez R;Badillo-Lopez C;Cerna-Cortes JF;Castillo-Ramirez I;Rivera-Gutierrez S;Helguera-Repetto AC;Aguilar D;Hernandez-Pando R;Samper S;Gonzalez-y-Merchand JA
墨西哥艾滋病毒感染者中结核分枝杆菌（MTb）和非结核分枝杆菌（NTM）感染的流行程度尚不清楚。本研究旨在确定墨西哥城hiv感染者中MTb和NTM菌株的频率，评估结核分枝杆菌复合菌株的基因型多样性，通过比色微孔板Alamar Blue法（MABA）确定其耐药谱，最后检测导致异烟肼（INH）和利福平（RIF）耐药的katG、rpoB和inhA基因突变。67株分枝杆菌中48株为结核分枝杆菌，9株为牛分枝杆菌，9株为鸟分枝杆菌，1株为胞内分枝杆菌。48株MTb菌株的IS6110-RFLP显示27个谱线。48株MTb菌株的spoligtyping产生21种模式，9株牛分枝杆菌菌株产生7种模式。发现了11种新的散斑型。在进行MIRU-VNTR时，从48株MTb菌株中共产生40种模式。19株（39.6%）结核分枝杆菌对一种或多种药物耐药。鉴定出1株（2.1%）耐多药菌株。在一株rpoB基因469密码子GAG→TCG （Glu→Ser）位点发现了一个新的rif抗性突变。这是对墨西哥hiv感染患者分离的分枝杆菌的首次分子分析，描述了该人群中不同分枝杆菌种类的流行情况。发现结核分枝杆菌菌株具有较高的遗传多样性。发现了新的spoligotypes和MIRU-VNTR模式以及与rif抗性相关的新突变。这些信息将有助于追踪艾滋病毒感染者体内不同的分枝杆菌种类，并监测这些微生物的传播，从而采取更适当的结核病控制措施。
The prevalence of infections with Mycobacterium tuberculosis (MTb) and nontuberculous mycobacteria (NTM) species in HIV-infected patients in Mexico is unknown. The aims of this study were to determine the frequency of MTb and NTM species in HIV-infected patients from Mexico City, to evaluate the genotypic diversity of the Mycobacterium tuberculosis complex strains, to determine their drug resistance profiles by colorimetric microplate Alamar Blue assay (MABA), and finally, to detect mutations present in katG, rpoB and inhA genes, resulting in isoniazid (INH) and rifampin (RIF) resistance. Of the 67 mycobacterial strains isolated, 48 were identified as MTb, 9 as M. bovis, 9 as M. avium and 1 as M. intracellulare. IS6110-RFLP of 48 MTb strains showed 27 profiles. Spoligotyping of the 48 MTb strains yielded 21 patterns, and 9 M. bovis strains produced 7 patterns. Eleven new spoligotypes patterns were found. A total of 40 patterns were produced from the 48 MTb strains when MIRU-VNTR was performed. Nineteen (39.6%) MTb strains were resistant to one or more drugs. One (2.1%) multidrug-resistant (MDR) strain was identified. A novel mutation was identified in a RIF-resistant strain, GAG → TCG (Glu → Ser) at codon 469 of rpoB gene. This is the first molecular analysis of mycobacteria isolated from HIV-infected patients in Mexico, which describe the prevalence of different mycobacterial species in this population. A high genetic diversity of MTb strains was identified. New spoligotypes and MIRU-VNTR patterns as well as a novel mutation associated to RIF-resistance were found. This information will facilitate the tracking of different mycobacterial species in HIV-infected individuals, and monitoring the spread of these microorganisms, leading to more appropriate measures for tuberculosis control.
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DOI：
10.1099/00221287-144-5-1189
发表时间：
1998-05-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Frothingham, R;Meeker-O'Connell, WA
通讯作者：
Meeker-O'Connell, WA
影响因子：
9.4
作者：
Allix, Caroline;Walravens, Karl;Fauville-Dufaux, Maryse
通讯作者：
Fauville-Dufaux, Maryse
影响因子：
9.4
作者：
Blackwood, KS;Wolfe, JN;Kabani, AM
通讯作者：
Kabani, AM
DOI：
10.1016/0962-8479(95)90533-2
发表时间：
1995-12-01
期刊：
TUBERCLE AND LUNG DISEASE
影响因子：
--
作者：
Das, S;Paramasivan, CN;Narayanan, PR
通讯作者：
Narayanan, PR
影响因子：
9.4
作者：
Bauer, J;Andersen, ÅB;Miörner, H
通讯作者：
Miörner, H