Stereotactic Body Radiotherapy and Conventional Radiotherapy Induce Cytoskeleton Extension and Enlargement of Cell Morphology in Non-Small Cell Lung Cancer.
Stereotactic Body Radiotherapy and Conventional Radiotherapy Induce Cytoskeleton Extension and Enlargement of Cell Morphology in Non-Small Cell Lung Cancer.
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DOI：
10.1177/15593258211064499
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发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Wang X;Lu Y;Qin Z;Guo H;Chen W;Ding T;Tang J;Zhang H
立体定向体部放射治疗（SBRT）目前广泛应用于癌症治疗。然而，SBRT与常规放疗（CRT）相比的生物学效应尚不清楚。细胞骨架在许多生物学过程和细胞生命活动中起着重要的作用。SBRT或CRT对非小细胞肺癌（NSCLC）细胞形态和细胞骨架结构的影响尚不清楚。根据生物等效剂量（BED）公式，我们设计了具有相同BED的SBRT和CRT分割方案。在辐射过程中捕获形态，并使用罗丹明鬼笔环肽免疫荧光来研究细胞骨架。采用乳酸脱氢酶试剂盒测定细胞膜通透性，western blot检测细胞骨架蛋白表达水平。SBRT或CRT后细胞形态和细胞骨架扩张，细胞膜通透性增加，细胞骨架蛋白水平稳定。不同剂量SBRT（10、20、30戈伊）诱导的细胞形态和细胞骨架增大相似。我们的研究结果表明，SBRT和CRT可以诱导细胞骨架重组和细胞形态的扩大（以不同的速度）在NSCLC。SBRT后的形态学和细胞骨架增大与剂量无关。
Stereotactic body radiotherapy (SBRT) is now widely used in cancer therapy. However, the biological effects of SBRT compared with conventional radiotherapy (CRT) are not clear. The cytoskeleton plays an important role in many biological processes and cellular life activities. The effects of SBRT or CRT on the morphology and cytoskeletal structure of non-small cell lung cancer (NSCLC) cells remain unknown. Based on the biologically equivalent dose (BED) formula, we designed SBRT and CRT fractionation regimens with the same BED. The morphology was captured during radiation, and rhodamine-phalloidin immunofluorescence was used to study the cytoskeleton. A lactate dehydrogenase assay kit was used to determine the cell membrane permeability, and western blot was used to detect the cytoskeleton protein expression levels. The morphology and cytoskeleton expanded after SBRT or CRT, with an increase in cell membrane permeability and stable cytoskeleton protein levels. Besides, different dose of SBRT (10,20,30 Gy) induce similar morphology and cytoskeleton enlargement. Our findings indicate that SBRT and CRT can induce cytoskeleton reorganization and the enlargement of cell morphology (at different rates) in NSCLC. The morphology and cytoskeleton enlargement after SBRT are dose independence.
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影响因子：
7.2
作者：
Pollard, Thomas D.;Goldman, Robert D.
通讯作者：
Goldman, Robert D.
影响因子：
5.6
作者：
Tani A;Chellini F;Giannelli M;Nosi D;Zecchi-Orlandini S;Sassoli C
通讯作者：
Sassoli C
影响因子：
2
作者：
Jiang, Ling;Xiong, Xiao-Peng;Ying, Hong-Mei
通讯作者：
Ying, Hong-Mei
影响因子：
5.3
作者：
Duverge, L.;Bondiau, P-Y;Castelli, J.
通讯作者：
Castelli, J.
影响因子：
5.7
作者：
Blanquie, Oriane;Bradke, Frank
通讯作者：
Bradke, Frank