Red (635 nm), Near-Infrared (808 nm) and Violet-Blue (405 nm) Photobiomodulation Potentiality on Human Osteoblasts and Mesenchymal Stromal Cells: A Morphological and Molecular In Vitro Study.
Red (635 nm), Near-Infrared (808 nm) and Violet-Blue (405 nm) Photobiomodulation Potentiality on Human Osteoblasts and Mesenchymal Stromal Cells: A Morphological and Molecular In Vitro Study.
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DOI：
10.3390/ijms19071946
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发表时间：
2018-07-03
影响因子：
5.6
通讯作者：
Sassoli C
中科院分区：
文献类型：
--
作者：
Tani A;Chellini F;Giannelli M;Nosi D;Zecchi-Orlandini S;Sassoli C
光生物调节（PBM）已在医学和牙科的不同领域用于骨再生目的，但矛盾的结果要求对其潜在的好处持怀疑态度。本体外研究比较了红色（635±5 nm）或近红外（808±10 nm）二极管激光器和紫蓝（405±5 nm）发光二极管在0.4 J/cm2能量密度的连续波下工作对人成骨细胞和间充质基质细胞（hMSC）活力、增殖、粘附和成骨分化的影响。PBM处理没有改变生存能力（PI/Syto16和MTS试验）。共聚焦免疫荧光和RT-PCR分析表明，两种细胞类型上的红色PBM (i)增加了富血管素簇、成骨标志物（Runx-2、碱性磷酸酶、骨桥蛋白）表达和矿化骨样结节结构沉积；（ii）在hMSCs上诱导应激纤维形成并上调增殖标志物Ki67的表达。有趣的是，成骨细胞对红光的反应是由Akt信号激活介导的，Akt信号激活似乎正向调节活性氧水平。紫外光照射后的细胞表现与未照射细胞基本相同，近红外照射后的细胞骨架组装、Runx-2表达和矿化模式发生改变。尽管在体外实验的限制下，本研究可能表明635 nm激光的PBM是促进/改善骨再生的潜在有效选择。
Photobiomodulation (PBM) has been used for bone regenerative purposes in different fields of medicine and dentistry, but contradictory results demand a skeptical look for its potential benefits. This in vitro study compared PBM potentiality by red (635 ± 5 nm) or near-infrared (NIR, 808 ± 10 nm) diode lasers and violet-blue (405 ± 5 nm) light-emitting diode operating in a continuous wave with a 0.4 J/cm2 energy density, on human osteoblast and mesenchymal stromal cell (hMSC) viability, proliferation, adhesion and osteogenic differentiation. PBM treatments did not alter viability (PI/Syto16 and MTS assays). Confocal immunofluorescence and RT-PCR analyses indicated that red PBM (i) on both cell types increased vinculin-rich clusters, osteogenic markers expression (Runx-2, alkaline phosphatase, osteopontin) and mineralized bone-like nodule structure deposition and (ii) on hMSCs induced stress fiber formation and upregulated the expression of proliferation marker Ki67. Interestingly, osteoblast responses to red light were mediated by Akt signaling activation, which seems to positively modulate reactive oxygen species levels. Violet-blue light-irradiated cells behaved essentially as untreated ones and NIR irradiated ones displayed modifications of cytoskeleton assembly, Runx-2 expression and mineralization pattern. Although within the limitations of an in vitro experimentation, this study may suggest PBM with 635 nm laser as potential effective option for promoting/improving bone regeneration.
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影响因子：
12.4
作者：
Chen Q;Shou P;Zheng C;Jiang M;Cao G;Yang Q;Cao J;Xie N;Velletri T;Zhang X;Xu C;Zhang L;Yang H;Hou J;Wang Y;Shi Y
通讯作者：
Shi Y
影响因子：
2.1
作者：
Chellini, Flaminia;Giannelli, Marco;Sassoli, Chiara
通讯作者：
Sassoli, Chiara
影响因子：
2.1
作者：
Ates, Gamze Bolukbasi;Can, Ayse Ak;Gulsoy, Murat
通讯作者：
Gulsoy, Murat
影响因子：
2.4
作者：
Chen, Chung-Huang;Hung, Huey-Shan;Hsu, Shan-Hui
通讯作者：
Hsu, Shan-Hui
影响因子：
2.4
作者：
Chellini, Flaminia;Sassoli, Chiara;Giannelli, Marco
通讯作者：
Giannelli, Marco