Antistaphylococcal nanocomposite films based on enzyme-nanotube conjugates.
Antistaphylococcal nanocomposite films based on enzyme-nanotube conjugates.
复制标题
DOI：
10.1021/nn100932t
复制
发表时间：
2010-07-27
期刊：
影响因子：
17.1
通讯作者：
Dordick JS
中科院分区：
文献类型：
--
作者：
Pangule RC;Brooks SJ;Dinu CZ;Bale SS;Salmon SL;Zhu G;Metzger DW;Kane RS;Dordick JS
耐甲氧西林金黄色葡萄球菌（MRSA）等耐药性病原体的感染是住院和死亡的主要原因之一。为了解决这个问题，我们设计了结合碳纳米管-酶缀合物的抗微生物涂层，其对耐药性病原体非常有效。具体而言，我们将碳纳米管与溶葡萄球菌酶（一种细胞壁降解酶）的缀合物结合到膜中，以赋予对金黄色葡萄球菌和表皮葡萄球菌的杀菌特性。我们制造和表征纳米复合材料含有不同的共轭制剂和酶负载。这些基于酶的复合物在杀死MRSA（在2小时内>99%）方面是高效的，而不会将酶释放到溶液中。此外，这些膜可重复使用，并且在干燥储存条件下稳定一个月。这种基于酶的膜制剂可用于防止医院环境中的各种常见表面上的病原性和耐药性微生物的生长。特别是含有这种抗微生物缀合物的聚合物和涂料膜可以有利于防止葡萄球菌特异性感染和生物污染的风险。
Infection with antibiotic-resistant pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) is one of the primary causes of hospitalizations and deaths. To address this issue, we have designed antimicrobial coatings incorporating carbon nanotube-enzyme conjugates that are highly effective against antibiotic–resistant pathogens. Specifically, we incorporated conjugates of carbon nanotubes with lysostaphin, a cell wall degrading enzyme, into films to impart bactericidal properties against Staphylococcus aureus and Staphylococcus epidermidis. We fabricated and characterized nanocomposites containing different conjugate formulations and enzyme loadings. These enzyme–based composites were highly efficient in killing MRSA (>99% within 2 h) without release of the enzyme into solution. Additionally, these films were reusable and stable under dry storage conditions for a month. Such enzyme–based film formulations may be used to prevent growth of pathogenic and antibiotic-resistant microorganisms on various common surfaces in hospital settings. Polymer and paint films containing such antimicrobial conjugates, in particular, could be advantageous to prevent risk of staphylococcal-specific infection and biofouling.
登录
查看更多内容
影响因子：
11.8
作者：
Klein E;Smith DL;Laxminarayan R
通讯作者：
Laxminarayan R
影响因子：
2
作者：
Dajcs, JJ;Thibodeaux, BA;O'Callaghan, RJ
通讯作者：
O'Callaghan, RJ
影响因子：
3.2
作者：
Gründling, A;Schneewind, O
通讯作者：
Schneewind, O
影响因子：
11.4
作者：
Baba, T;Schneewind, O
通讯作者：
Schneewind, O
影响因子：
2.7
作者：
Lin, J;Tiller, JC;Klibanov, AM
通讯作者：
Klibanov, AM