Regulation of Fig (Ficus carica L.) Fruit Color: Metabolomic and Transcriptomic Analyses of the Flavonoid Biosynthetic Pathway.
Regulation of Fig (Ficus carica L.) Fruit Color: Metabolomic and Transcriptomic Analyses of the Flavonoid Biosynthetic Pathway.
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无花果（Ficus carica L.）果实颜色的调节：类黄酮生物合成途径的代谢组学和转录组学分析
DOI：
10.3389/fpls.2017.01990
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发表时间：
2017
影响因子：
5.6
通讯作者：
Ma H
中科院分区：
文献类型：
--
作者：
Wang Z;Cui Y;Vainstein A;Chen S;Ma H
对无花果品种“绿皮”及其颜色突变体“紫皮”进行了代谢组学和转录组学分析。鉴定出5种代谢物和22种代谢物在幼龄和成熟期的果皮中含量显著不同。花青素o -丙二醇己糖在成熟的紫色果皮中含量增加了3992倍，这是无花果果实中首次发现的一种主要花青素；花青素3- o -葡萄糖苷、花青素o -丙二酰己糖苷和花青素3,5- o -二葡糖苷上调100倍，揭示了紫色突变背后的花青素。除了可见的差异外，成熟的“紫皮”与“绿皮”相比，无色黄酮类原花青素B1、木黄素-3′、7-二- o -葡萄糖苷、表儿茶素和槲皮素-3- o -鼠李糖苷的积累也非常显著。在幼龄阶段，突变体中只有花青素o -丙二醇己糖、花青素o -丙二醇己糖、o -己糖和橙皮素的表达上调了几倍。转录组分析显示，与“绿皮”相比，“紫皮”幼体中苯丙素和类黄酮生物合成途径酶编码基因的表达呈下调趋势，而成熟突变体中几乎所有类黄酮和花青素途径组分及相关转录因子的表达均显著上调。讨论了R2R3-MYB转录因子在颜色形态突变中的作用及其与反转录转座子活性的可能关系。此外，在成熟突变体中发现了小热休克蛋白基因的大规模上调。这是首次揭示无花果单一园艺属性突变的综合代谢组和转录组网络变化及其对果实营养和品质的深远影响。
Combined metabolomic and transcriptomic analyses were carried out with fig cultivar Green Peel and its color mutant “Purple Peel.” Five and twenty-two metabolites were identified as having significantly different contents between fruit peels of the two cultivars at young and mature stages, respectively. Cyanidin O-malonylhexoside demonstrated a 3,992-fold increase in the mature purple peel, the first identification of a major cyanidin in fig fruit; cyanidin 3-O-glucoside, cyanidin O-malonylhexoside O-hexoside and cyanidin-3,5-O-diglucoside were upregulated 100-fold, revealing the anthocyanins underlying the purple mutation. Beyond the visible differences, there was very significant accumulation of the colorless flavonoids procyanidin B1, luteolin-3′,7-di-O-glucoside, epicatechin and quercetin-3-O-rhamnoside in the mature “Purple Peel” compared to “Green Peel.” At the young stage, only cyanidin O-malonylhexoside, cyanidin O-malonylhexoside O-hexoside and esculetin were upregulated a few fold in the mutant. Transcriptome analysis revealed a downregulated expression trend of genes encoding phenylpropanoid and flavonoid biosynthetic pathway enzyme in the young “Purple Peel” compared to the young “Green Peel,” whereas significant and simultaneous upregulation was revealed in almost all of the flavonoid and anthocyanin pathway components and relevant transcription factors in the mature-stage mutant. The role of R2R3-MYB transcription factors in the color morph mutation and its possible relation to the activity of retrotransposons are discussed. Moreover, large-scale upregulation of small heat-shock protein genes was found in the mature mutant. This is the first work to reveal comprehensive metabolome and transcriptome network changes underlying a fig mutation in a single horticultural attribute, and its profound effects on fruit nutrition and quality.
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DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
北极芥末花颜色多态性由花瓣特异性下调在花青素生物合成途径的阈值下控制。
影响因子：
3.7
作者：
Dick CA;Buenrostro J;Butler T;Carlson ML;Kliebenstein DJ;Whittall JB
通讯作者：
Whittall JB
影响因子：
6.9
作者：
Han Y;Vimolmangkang S;Soria-Guerra RE;Korban SS
通讯作者：
Korban SS
影响因子：
4.3
作者：
Duenas, Montserrat;Perez-Alonso, Jose Joaquin;Escribano-Bailon, Teresa
通讯作者：
Escribano-Bailon, Teresa
影响因子：
7.4
作者：
Griesser, Markus;Hoffmann, Thomas;Schwab, Wilfried
通讯作者：
Schwab, Wilfried