A simple and sensitive method for the demonstration of norepinephrine-storing adrenomedullary chromaffin cells.
A simple and sensitive method for the demonstration of norepinephrine-storing adrenomedullary chromaffin cells.
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DOI：
10.1007/s00418-012-0942-3
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发表时间：
2012-07
影响因子：
2.3
通讯作者：
Kamitani, Tetsu
中科院分区：
文献类型：
--
作者：
Khan, Mohammad Badruzzaman;Lee, Byung Rho;Kamitani, Tetsu
肾上腺髓质是一种神经内分泌组织，其中存在储存儿茶酚胺的嗜铬细胞。嗜铬细胞来源于神经嵴细胞，并明显分化为肾上腺素储存细胞和去甲肾上腺素储存细胞两类。使用组织化学或免疫染色方法，两种类型的嗜铬细胞已被区分。然而，这些方法的困难和/或缺陷在一定程度上限制了e -存储细胞和ne -存储细胞差异功能的研究进展。在这里，我们展示了一种新的方法来区分这两种细胞类型。我们发现，在pH为6的柠檬酸缓冲液处理后，小鼠和大鼠肾上腺髓细胞被Harris苏木精异质染色。苏木精染色的细胞簇呈酪氨酸羟化酶阳性，酪氨酸羟化酶是一种参与儿茶酚胺生物合成的酶。此外，细胞簇中苯乙醇胺- n -甲基转移酶呈阴性，该酶负责NE到E的转化，并在储存E的染色质细胞中表达。此外，我们发现用苏木精染色的细胞团也可以用硝基蓝四氮唑在pH 11下染色，使用Hopsu和Mäkinen的方法对ne储存的染色质细胞进行染色。这些观察结果表明，在pH为6的柠檬酸缓冲液处理后，储存ne的嗜铬细胞的细胞质被苏木精特异性染色。这种方法将使我们能够促进嗜铬细胞的细胞类型特异性研究。事实上，该方法显示α-synuclein在储存e -染色质细胞中选择性表达，而在储存ne -染色质细胞中不表达。
The medulla of the adrenal gland is a neuroendocrine tissue in which catecholamine-storing chromaffin cells exist. The chromaffin cells are derived from neural crest cells and distinctly differentiated into two types of cells, epinephrine (E) (adrenaline)-storing and norepinephrine (NE) (noradrenaline)-storing cells. Using histochemical or immunostaining methods, the two types of chromaffin cells have been differentially distinguished. However, difficulties and/or drawbacks of the procedures have somewhat restricted the progress of research in differential functions of E-storing and NE-storing cells. Here, we show a new method for the differential demonstration of these two cell types. We found that mouse and rat adrenomedullary cells are heterogeneously stained with Harris hematoxylin after treatment with citrate buffer at pH 6. The cell clusters stained with hematoxylin were positive for tyrosine hydroxylase, which is an enzyme involved in catecholamine biosynthesis. Furthermore, the cell clusters were negative for phenylethanolamine-N-methyl transferase, which is an enzyme responsible for the conversion from NE to E and expresses in E-storing chromaffin cells. Moreover, we found that the cell clusters stained with hematoxylin can also be stained with nitroblue tetrazolium at pH 11, using Hopsu and Mäkinen’s method by which NE-storing chromaffin cells are stained. These observations indicate that the cytoplasm of NE-storing chromaffin cells is specifically stained with hematoxylin after treatment with citrate buffer at pH 6. This method will allow us to facilitate cell-type specific research of chromaffin cells. Indeed, this method revealed that α-synuclein selectively expresses in E-storing chromaffin cells, but not in NE-storing chromaffin cells.
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影响因子：
6.1
作者：
Tanji K;Kamitani T;Mori F;Kakita A;Takahashi H;Wakabayashi K
通讯作者：
Wakabayashi K
影响因子：
3.7
作者：
Matsuo Y;Kamitani T
通讯作者：
Kamitani T
DOI：
10.1007/bf02135767
发表时间：
1971-01-01
期刊：
EXPERIENTIA
影响因子：
--
作者：
GOLDSTEIN, M;FUXE, K;JOH, TH
通讯作者：
JOH, TH
DOI：
10.1016/s0165-1838(96)00100-2
发表时间：
1996-12-14
期刊：
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM
影响因子：
--
作者：
Hou, XE;Dahlstrom, A
通讯作者：
Dahlstrom, A
影响因子：
2.8
作者：
Phillips, JK;Dubey, R;Lipski, J
通讯作者：
Lipski, J