Mass production of genetically modified Aedes aegypti for field releases in Brazil.
Mass production of genetically modified Aedes aegypti for field releases in Brazil.
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DOI：
10.3791/3579
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发表时间：
2014-01-04
期刊：
影响因子：
--
通讯作者：
Capurro ML
中科院分区：
文献类型：
--
作者：
Carvalho DO;Nimmo D;Naish N;McKemey AR;Gray P;Wilke AB;Marrelli MT;Virginio JF;Alphey L;Capurro ML
人们正在寻求新的技术和方法来赢得与蚊子的斗争。分子技术的最新进展导致了基于不育昆虫技术（SIT）1-3的新的和创新的蚊子控制方法的发展。一种被称为RIDL（释放携带显性致死基因的昆虫）4的控制方法是基于SIT，但使用遗传方法来消除辐射灭菌的需要5 -8。Ae.在大开曼岛9、10成功地进行了实地试验;世界其他国家正在计划或正在进行进一步的实地使用。已经在几种昆虫物种中建立了昆虫的大规模饲养，并且达到每周数十亿的水平。然而，在蚊子中，饲养通常以小得多的规模进行，大多数大规模饲养是在20世纪70年代和80年代进行的。对于一个RIDL程序，它是可取的释放尽可能少的女性，因为他们咬和传播疾病。在大规模饲养计划中，有几个阶段来生产要释放的雄性：产卵，饲养蛋直到化蛹，然后在释放之前将雄性从雌性中分离出来。然后将这些雄性用于RIDL控制程序，作为幼鼠或成年人释放11，12。为了使用RIDL抑制蚊子种群，需要饲养大量高质量的雄性成虫13，14。以下描述了大量饲养Ae的RIDL菌株OX 513 A的方法。aegypti 8，用于释放，并涵盖了用于控制程序的卵生产和大量饲养RIDL雄性所需的技术。
New techniques and methods are being sought to try to win the battle against mosquitoes. Recent advances in molecular techniques have led to the development of new and innovative methods of mosquito control based around the Sterile Insect Technique (SIT)1-3. A control method known as RIDL (Release of Insects carrying a Dominant Lethal)4, is based around SIT, but uses genetic methods to remove the need for radiation-sterilization5-8. A RIDL strain of Ae. aegypti was successfully tested in the field in Grand Cayman9,10; further field use is planned or in progress in other countries around the world. Mass rearing of insects has been established in several insect species and to levels of billions a week. However, in mosquitoes, rearing has generally been performed on a much smaller scale, with most large scale rearing being performed in the 1970s and 80s. For a RIDL program it is desirable to release as few females as possible as they bite and transmit disease. In a mass rearing program there are several stages to produce the males to be released: egg production, rearing eggs until pupation, and then sorting males from females before release. These males are then used for a RIDL control program, released as either pupae or adults11,12. To suppress a mosquito population using RIDL a large number of high quality male adults need to be reared13,14. The following describes the methods for the mass rearing of OX513A, a RIDL strain of Ae. aegypti 8, for release and covers the techniques required for the production of eggs and mass rearing RIDL males for a control program.
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影响因子：
3.7
作者：
Bargielowski I;Alphey L;Koella JC
通讯作者：
Koella JC
DOI：
10.1093/aesa/50.1.104
发表时间：
1957-01-01
期刊：
ANN ENT SOC AMER
影响因子：
--
作者：
JONES, JACK COLVARD
通讯作者：
JONES, JACK COLVARD
DOI：
10.1590/s0074-02762003000500020
发表时间：
2003-07-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Macoris, Maria de Lourdes G;Andrighetti, Maria Teresa M;Bracco, José Eduardo
通讯作者：
Bracco, José Eduardo
影响因子：
46.9
作者：
Harris, Angela F.;Nimmo, Derric;Alphey, Luke
通讯作者：
Alphey, Luke
DOI：
10.1073/pnas.1000251107
发表时间：
2010-03-09
影响因子：
11.1
作者：
Fu, Guoliang;Lees, Rosemary S.;Alphey, Luke
通讯作者：
Alphey, Luke