Rapid Gene Evolution in an Ancient Post-transcriptional and Translational Regulatory System Compensates for Meiotic X Chromosomal Inactivation.
Rapid Gene Evolution in an Ancient Post-transcriptional and Translational Regulatory System Compensates for Meiotic X Chromosomal Inactivation.
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一个古老的转录后和翻译调控系统中的快速基因进化补偿了减数分裂X染色体失活。
DOI：
10.1093/molbev/msab296
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发表时间：
2022-01-07
影响因子：
10.7
通讯作者：
Long M
中科院分区：
文献类型：
--
作者：
Xia S;Ventura IM;Blaha A;Sgromo A;Han S;Izaurralde E;Long M
传统上认为，保守的途径进化缓慢，很少参与基因进化。然而，最近观察到，年轻的基因可以在基本的生物过程中发挥基本功能，例如发育和繁殖。目前尚不清楚新复制的基因如何整合到祖先网络中，并重塑重要功能的保守途径。在这里，我们研究了最近在果蝇中进化的两个常染色体基因的起源和功能：Poseidon和Zeus，这两个基因是通过X-连锁CAF40的RNA复制而产生的，CAF40是保守的CCR4-Not Deadenylase复合体的一个亚单位，参与转录后和翻译调控。基因敲除和敲除分析表明，这两个基因迅速进化出在生存能力和男性生育能力方面至关重要的功能。此外，我们的转录组分析表明，这三个基因在数百个基因的表达中具有广泛而独特的影响，几乎一半的差异表达基因仅被一个旁对基因干扰，而不是其他基因。免疫共沉淀和系链分析表明，CAF40 Paralog Poseidon保持了与CCR4-Not Deadenylase复合体相互作用的能力，并可能参与转录后mRNA的调节。在古老的转录后和翻译调控系统中，基因的快速进化可能是由于性染色体的进化，以补偿果蝇减数分裂X染色体失活(MXCI)。
It is conventionally assumed that conserved pathways evolve slowly with little participation of gene evolution. Nevertheless, it has been recently observed that young genes can take over fundamental functions in essential biological processes, for example, development and reproduction. It is unclear how newly duplicated genes are integrated into ancestral networks and reshape the conserved pathways of important functions. Here, we investigated origination and function of two autosomal genes that evolved recently in Drosophila: Poseidon and Zeus, which were created by RNA-based duplications from the X-linked CAF40, a subunit of the conserved CCR4–NOT deadenylase complex involved in posttranscriptional and translational regulation. Knockdown and knockout assays show that the two genes quickly evolved critically important functions in viability and male fertility. Moreover, our transcriptome analysis demonstrates that the three genes have a broad and distinct effect in the expression of hundreds of genes, with almost half of the differentially expressed genes being perturbed exclusively by one paralog, but not the others. Co-immunoprecipitation and tethering assays show that the CAF40 paralog Poseidon maintains the ability to interact with the CCR4–NOT deadenylase complex and might act in posttranscriptional mRNA regulation. The rapid gene evolution in the ancient posttranscriptional and translational regulatory system may be driven by evolution of sex chromosomes to compensate for the meiotic X chromosomal inactivation (MXCI) in Drosophila.
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影响因子：
64.8
作者：
Clark, Andrew G.;Eisen, Michael B.;MacCallum, Iain
通讯作者：
MacCallum, Iain
影响因子：
4.5
作者：
Ding Y;Zhao L;Yang S;Jiang Y;Chen Y;Zhao R;Zhang Y;Zhang G;Dong Y;Yu H;Zhou Q;Wang W
通讯作者：
Wang W
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1007/978-3-319-46503-6_13
发表时间：
2017-01-01
期刊：
MACROMOLECULAR PROTEIN COMPLEXES: STRUCTURE AND FUNCTION
影响因子：
--
作者：
Collart, Martine A.;Panasenko, Olesya O.
通讯作者：
Panasenko, Olesya O.
影响因子：
7
作者：
Carelli FN;Hayakawa T;Go Y;Imai H;Warnefors M;Kaessmann H
通讯作者：
Kaessmann H