Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study.
Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study.
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DOI：
10.3390/cells10092284
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发表时间：
2021-09-02
期刊：
影响因子：
6
通讯作者：
Griffin DK
中科院分区：
文献类型：
--
作者：
Silvestri G;Canedo-Ribeiro C;Serrano-Albal M;Labrecque R;Blondin P;Larmer SG;Marras G;Tutt DAR;Handyside AH;Farré M;Sinclair KD;Griffin DK
每年全世界大约有100万个体外培养（IVP）牛胚胎被移植，以提高遗传增益率。最先进的计划还通过SNP基因分型和基因组估计育种值（gebv）的计算在胚胎阶段应用基因组选择。然而，很大比例的牛胚胎不能怀孕。在这里，我们证明了对收集的gebv SNP数据的进一步分析可以有效地从池中去除非整倍体胚胎，提高每次胚胎移植（ET）的活产率。采用三种非整倍体植入前基因检测（PGT-A）方法，我们对1713个牛胚泡进行了盲法回顾性分析。我们的研究结果表明，非整倍体胚胎有5.8%的机会怀孕，5.0%的机会活产。相比之下，整倍体胚胎为59.6%和46.7% （p < 0.0001）。PGT-A可使总妊娠率和活产率分别提高7.5%和5.8% （p < 0.0001）。更详细的分析揭示了供体、染色体、分期、分级和性别特异性的错误率。值得注意的是，我们发现XY胚胎的非整倍性发生率明显较高，并且与人类一样，检测到母体减数分裂I错误的优势。我们的数据强烈支持在牛IVP计划中使用PGT-A。
Approximately one million in vitro produced (IVP) cattle embryos are transferred worldwide each year as a way to improve the rates of genetic gain. The most advanced programmes also apply genomic selection at the embryonic stage by SNP genotyping and the calculation of genomic estimated breeding values (GEBVs). However, a high proportion of cattle embryos fail to establish a pregnancy. Here, we demonstrate that further interrogation of the SNP data collected for GEBVs can effectively remove aneuploid embryos from the pool, improving live births per embryo transfer (ET). Using three preimplantation genetic testing for aneuploidy (PGT-A) approaches, we assessed 1713 cattle blastocysts in a blind, retrospective analysis. Our findings indicate aneuploid embryos have a 5.8% chance of establishing a pregnancy and a 5.0% chance of given rise to a live birth. This compares to 59.6% and 46.7% for euploid embryos (p < 0.0001). PGT-A improved overall pregnancy and live birth rates by 7.5% and 5.8%, respectively (p < 0.0001). More detailed analyses revealed donor, chromosome, stage, grade, and sex-specific rates of error. Notably, we discovered a significantly higher incidence of aneuploidy in XY embryos and, as in humans, detected a preponderance of maternal meiosis I errors. Our data strongly support the use of PGT-A in cattle IVP programmes.
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DOI：
10.1093/bioinformatics/btx346
发表时间：
2017-10-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Gel B;Serra E
通讯作者：
Serra E
DOI：
10.1016/j.tig.2017.04.001
发表时间：
2017-07
期刊：
Trends in genetics : TIG
影响因子：
--
作者：
McCoy RC
通讯作者：
McCoy RC
影响因子：
1.7
作者：
Hornak, Miroslav;Kubicek, David;Rubes, Jiri
通讯作者：
Rubes, Jiri
影响因子：
2.8
作者：
MILLER, GF;GLIEDT, DW;RORIE, RW
通讯作者：
RORIE, RW
DOI：
10.1534/g3.113.007377
发表时间：
2013-10-03
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Baryshnikova A;VanderSluis B;Costanzo M;Myers CL;Cha RS;Andrews B;Boone C
通讯作者：
Boone C